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Repression of Cell-Cell Fusion by Components of the C. elegans Vacuolar ATPase Complex
- Source :
- Developmental Cell. (5):787-794
- Publisher :
- Elsevier Inc.
-
Abstract
- Summary Cell-cell fusion initiates fertilization, sculpts tissues during animal development, reprograms stem cells to new differentiated states, and may be a key step in cancer progression. While cell fusion is tightly regulated, the mechanisms that limit fusion to appropriate partners are unknown. Here, we report that the fus-1 gene is essential to repress fusion of epidermal cells in C. elegans : in severe fus-1 mutants, all epidermal cells, except the lateral seam cells, inappropriately fuse into a single large syncytium. This hyperfusion requires EFF-1, an integral membrane protein essential for fusion of epidermal cells into discrete syncytia. FUS-1 is localized to the apical plasma membrane in all epidermal cells potentiated to undergo fusion, whereas it is virtually undetectable in nonfusing seam cells. fus-1 encodes the e subunit of the vacuolar H + -ATPase (V-ATPase), and loss of other V-ATPase subunits also causes widespread hyperfusion. These findings raise the possibility of manipulating cell fusion by altering V-ATPase activity.
- Subjects :
- Vacuolar Proton-Translocating ATPases
Protein subunit
ATPase
Mutant
Biology
General Biochemistry, Genetics and Molecular Biology
Cell Fusion
03 medical and health sciences
0302 clinical medicine
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Molecular Biology
Integral membrane protein
Genes, Helminth
030304 developmental biology
0303 health sciences
Syncytium
Cell fusion
Membrane Glycoproteins
Cell Biology
Cell biology
Protein Subunits
Biochemistry
Mutation
biology.protein
Stem cell
030217 neurology & neurosurgery
Fusion mechanism
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 15345807
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Developmental Cell
- Accession number :
- edsair.doi.dedup.....2baae4f14c428ca6dbf3e9fdec96f9aa
- Full Text :
- https://doi.org/10.1016/j.devcel.2005.02.018