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Mesenchymal stem cells reduce alcoholic hepatitis in mice via suppression of hepatic neutrophil and macrophage infiltration, and of oxidative stress
- Source :
- PLoS ONE, Vol 15, Iss 2, p e0228889 (2020), PLoS ONE
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Mesenchymal stem cells (MSCs) are a population of pluripotent cells that have been tested for the treatment of many inflammatory diseases. It remains unclear whether MSCs were effective in treating mice with alcoholic hepatitis (AH) and its underlying mechanism. In the present study, MSCs were isolated from bone marrow of 4–6 week-old C57BL/6N male mice. AH was induced in female mice by chronic-binge ethanol feeding for 10 days. Intraperitoneal (i.p.) transplantation of MSCs or saline were performed in mice on day 10. Blood samples and hepatic tissues were harvested on day 11. Biochemical, liver histological and flow cytometric analyses were performed. Compared to the control mice, the AH mice had significantly increased liver/body weight ratio, serum alanine aminotransferase (ALT) and aspartate aminotransferases (AST), hepatic total cholesterol (TC), triglyceride (TG), malondialdehyde (MDA), hepatic neutrophil and macrophage infiltration (P
- Subjects :
- Male
0301 basic medicine
Steatosis
Neutrophils
Pathology and Laboratory Medicine
medicine.disease_cause
Cytopathology
White Blood Cells
Mice
chemistry.chemical_compound
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
education.field_of_study
Multidisciplinary
Stem Cells
Liver Diseases
Fatty liver
Cell Differentiation
Animal Models
Malondialdehyde
medicine.anatomical_structure
Experimental Organism Systems
Liver
Neutrophil Infiltration
Medicine
Female
030211 gastroenterology & hepatology
Cellular Types
Research Article
medicine.medical_specialty
Immune Cells
Science
Immunology
Population
Alcoholic hepatitis
Surgical and Invasive Medical Procedures
Mouse Models
Gastroenterology and Hepatology
Research and Analysis Methods
Mesenchymal Stem Cell Transplantation
Digestive System Procedures
03 medical and health sciences
Model Organisms
Internal medicine
medicine
Animals
education
Transplantation
Blood Cells
Hepatitis, Alcoholic
business.industry
Macrophages
Mesenchymal stem cell
Biology and Life Sciences
Mesenchymal Stem Cells
Cell Biology
Organ Transplantation
Lipid Metabolism
medicine.disease
Liver Transplantation
Fatty Liver
Mice, Inbred C57BL
Oxidative Stress
Disease Models, Animal
030104 developmental biology
Endocrinology
chemistry
Anatomical Pathology
Animal Studies
Bone marrow
business
Oxidative stress
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....2ba1f36acd907f9ddd0d9399ecab9710