Comprehensive and efficient analyses of T cell receptors for cancer antigens in peptide-vaccinated patients using a single cell-based gene cloning system

Obtaining T cell receptor repertoires specific for cancer antigens in individuals is of great significance in terms of their relationships with the clinical courses and therapeutic application such as adoptive transfer. However, full analyses of T cell receptor (TCR) sequences have been difficult because of the inefficiency of T cell cloning, heterogeneity of T cells or the heterodimeric structures of TCRs. Here, we revealed complete landscapes of cancer antigen-specific TCRs using a novel TCR cloning system; hTEC10 (human TCR efficient cloning within 10 days) [1].