The biocompatibility of silver-containing Na2O CaO 2SiO2 glass prepared by sol-gel method: in vitro studies

This study was performed to determine the in vitro biocompatibility of a bioactive, antibacterial silver based gel. A human osteoblast-like cell line (HOS TE85) was used to test cytocompatibility in the presence of eluants and the biological response in direct contact with Na2O·CaO·2SiO2 gel (containing 0.5050 wt % Ag2O prepared by a sol–gel process) sterilized by two different methods; autoclave and γ-irradiation. MTT assay was used for the indirect evaluation of eluant toxicity and alamar Blue™ was performed to assess the biological response, which included cell viability and proliferation, and the results showed that these materials were cytocompatible. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2010