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Neurotoxic Zanthoxylum chalybeum root constituents invoke mosquito larval growth retardation through ecdysteroidogenic CYP450s transcriptional perturbations
- Source :
- Pesticide biochemistry and physiology. 178
- Publication Year :
- 2021
-
Abstract
- Intracellular effects exerted by phytochemicals eliciting insect growth-retarding responses during vector control intervention remain largely underexplored. We studied the effects of Zanthoxylum chalybeum Engl. (Rutaceae) (ZCE) root derivatives against malaria (Anopheles gambiae) and arbovirus vector (Aedes aegypti) larvae to decipher possible molecular targets. We report dose-dependent biphasic effects on larval response, with transient exposure to ZCE and its bioactive fraction (ZCFr.5) inhibiting acetylcholinesterase (AChE) activity, inducing larval lethality and growth retardation at sublethal doses. Half-maximal lethal concentrations (LC50) for ZCE against An. gambiae and Ae. aegypti larvae after 24-h exposure were 9.00 ppm and 12.26 ppm, respectively. The active fraction ZCFr.5 exerted LC50 of 1.58 ppm and 3.21 ppm for An. gambiae and Ae. aegypti larvae, respectively. Inhibition of AChE was potentially linked to larval toxicity afforded by 2-tridecanone, palmitic acid (hexadecanoic acid), linoleic acid ((Z,Z)-9,12-octadecadienoic acid), sesamin, β-caryophyllene among other compounds identified in the bioactive fraction. In addition, the phenotypic larval retardation induced by ZCE root constituents was exerted through transcriptional modulation of ecdysteroidogenic CYP450 genes. Collectively, these findings provide an explorative avenue for developing potential mosquito control agents from Z. chalybeum root constituents.
- Subjects :
- Zanthoxylum
Insecticides
Mosquito Control
Aché
Health, Toxicology and Mutagenesis
Anopheles gambiae
Linoleic acid
Aedes aegypti
Mosquito Vectors
Palmitic acid
chemistry.chemical_compound
Sesamin
Aedes
parasitic diseases
Animals
Growth Disorders
Larva
biology
Plant Extracts
fungi
General Medicine
biology.organism_classification
Acetylcholinesterase
language.human_language
Culex
chemistry
Biochemistry
language
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 10959939
- Volume :
- 178
- Database :
- OpenAIRE
- Journal :
- Pesticide biochemistry and physiology
- Accession number :
- edsair.doi.dedup.....2b5bc9ac20ceb448bdaf2e4ae16b6bda