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Reduced chromatin accessibility correlates with resistance to Notch activation
- Source :
- van den Ameele, J, Krautz, R, Cheetham, S W, Donovan, A P A, Llorà-Batlle, O, Yakob, R & Brand, A H 2022, ' Reduced chromatin accessibility correlates with resistance to Notch activation ', Nature Communications, vol. 13, no. 1, 2210 . https://doi.org/10.1038/s41467-022-29834-z
- Publication Year :
- 2022
- Publisher :
- Springer Science and Business Media LLC, 2022.
-
Abstract
- Funder: Royal Society; doi: https://doi.org/10.13039/501100000288<br />Funder: Herchel Smith Fund<br />The Notch signalling pathway is a master regulator of cell fate transitions in development and disease. In the brain, Notch promotes neural stem cell (NSC) proliferation, regulates neuronal migration and maturation and can act as an oncogene or tumour suppressor. How NOTCH and its transcription factor RBPJ activate distinct gene regulatory networks in closely related cell types in vivo remains to be determined. Here we use Targeted DamID (TaDa), requiring only thousands of cells, to identify NOTCH and RBPJ binding in NSCs and their progeny in the mouse embryonic cerebral cortex in vivo. We find that NOTCH and RBPJ associate with a broad network of NSC genes. Repression of NSC-specific Notch target genes in intermediate progenitors and neurons correlates with decreased chromatin accessibility, suggesting that chromatin compaction may contribute to restricting NOTCH-mediated transactivation.
- Subjects :
- Multidisciplinary
Receptors, Notch
article
45/22
General Physics and Astronomy
45/23
Cell Differentiation
General Chemistry
Chromatin
General Biochemistry, Genetics and Molecular Biology
Mice
Neural Stem Cells
nervous system
631/136/142
13/51
Animals
64/60
45/100
14/19
631/136/368
Signal Transduction
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....2b37012fb0cbf9d4bd9e6aff7b0dfbc3