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Broad-Spectrum and Gram-Negative-Targeting Antibiotics Differentially Regulate Antibody Isotype Responses to Injected Vaccines
- Source :
- Vaccines, Vol 9, Iss 1240, p 1240 (2021), Vaccines, Volume 9, Issue 11
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Antibiotics are extensively used worldwide for the treatment of common infections by agents such as E. coli and Salmonella. They also represent the most common cause of alteration of the microbiota in people. We addressed whether broad-spectrum and Gram-negative-targeting antibiotics differentially regulate systemic and mucosal immune responses to vaccines. Antibiotics treatment enhances serum IgG1 responses in mice immunized systemically with a model polyvalent vaccine. This increase was not seen for other IgG subclasses and was dependent on the immunogenicity of vaccine antigens. The broad-spectrum antibiotic cocktail also enhanced serum IgA responses. Interestingly, both the broad spectrum and the antibiotic targeting Gram-negative bacteria enhanced the number of IgA antibody secreting cells in the intestinal lamina propria. This effect was unlikely to be due to an increase in cells expressing gut-homing receptors (i.e., CCR9 and α4β7) in peripheral tissues. On the other hand, the microbiome in mice treated with antibiotics was characterized by an overall reduction of the number of firmicutes. Furthermore, Bacteroidetes were increased by either treatment, and Proteobacteria were increased by the broad-spectrum antibiotics cocktail. Thus, immunoglobulin isotype and subclass responses are differentially regulated by oral antibiotics treatment and the gut microbiota shapes mucosal antibody responses after systemic immunization.
- Subjects :
- Pharmacology
biology
medicine.drug_class
Polyvalent Vaccine
Immunogenicity
Immunology
Antibiotics
Gut flora
vaccines
biology.organism_classification
Article
antibiotics
Infectious Diseases
Antibody Isotype
Immunization
IgA responses
Drug Discovery
medicine
Medicine
Pharmacology (medical)
Microbiome
Bacteria
Subjects
Details
- Language :
- English
- Volume :
- 9
- Issue :
- 1240
- Database :
- OpenAIRE
- Journal :
- Vaccines
- Accession number :
- edsair.doi.dedup.....2b337535343b518167798773e7ac40cc