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Soy peptides enhance heterologous membrane protein productivity during the exponential growth phase of Saccharomyces cerevisiae
- Source :
- Bioscience, biotechnology, and biochemistry. 76(3)
- Publication Year :
- 2012
-
Abstract
- In this study, the production of eight G protein-coupled receptors by Saccharomyces cerevisiae was compared using two types of media, one of which contained soy peptides and the other free amino acids. Yeast cell growth improved in the medium with soy peptides, and the expression levels of six of the receptors increased during the exponential phase by an average of 2.3-fold as against the free amino acid-based medium. The enhancement of protein expression by soy peptides can be explained by alleviation of metabolite stress due to amino acid source depletion caused by heterologous protein expression.
- Subjects :
- Saccharomyces cerevisiae
Heterologous
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
Receptors, G-Protein-Coupled
Exponential growth
Humans
Receptor
Molecular Biology
Cell Proliferation
chemistry.chemical_classification
biology
Cell growth
Organic Chemistry
General Medicine
biology.organism_classification
Yeast
Peptide Fragments
Amino acid
Membrane protein
chemistry
Soybean Proteins
Biotechnology
Subjects
Details
- ISSN :
- 13476947
- Volume :
- 76
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Accession number :
- edsair.doi.dedup.....2b334423c2eafd71f7b768b72ebee480