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Dual Functionalized Injectable Hybrid Extracellular Matrix Hydrogel for Burn Wounds
- Source :
- Bankoti, K, Rameshbabu, A P, Datta, S, Goswami, P, Roy, M, Das, D, Ghosh, S K, Das, A K, Mitra, A, Pal, S, Maulik, D, Su, B, Ghosh, P, Basu, B & Dhara, S 2021, ' Dual Functionalized Injectable Hybrid Extracellular Matrix Hydrogel for Burn Wounds ', Biomacromolecules, vol. 22, no. 2, pp. 514–533 . https://doi.org/10.1021/acs.biomac.0c01400
- Publication Year :
- 2020
- Publisher :
- American Chemical Society (ACS), 2020.
-
Abstract
- Low strength and rapid biodegradability of Acellular Dermal Matrix (ADM) restrict wider clinical application as rapid cell delivery platform in situ, for management of burn wounds. Herein, extracted ADM was modified by dual cross-linking approach with ionic crosslinking using chitosan (CTS) and covalent cross-linking using an iodine modified 2, 5-dihydro-2,5-dimethoxy-furan (DHF-I) cross-linker; termed as CsADM-Cl. In addition, inherent growth factors and cytokines were found to be preserved in the CsADM-Cl, irrespective of ionic/covalent crosslinking. CsADM-Cl demonstrated improvement in post crosslinking stiffness with decreased biodegradation rate. This hybrid crosslinked hydrogel supported adhesion, proliferation, and migration of human foreskin derived fibroblasts (HFCs) and keratinocytes (HKC). Also, the angiogenic potential of CsADM-Cl was manifested by chick chorioallantoic membrane (CAM) assay. CsADM-Cl showed excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Moreover, CsADM-Cl treated full thickness (FT) burn wounds demonstrated rapid healing marked with superior angiogenesis, well-defined dermal-epidermal junctions (DEJ), mature basket weave collagen deposition, and development of more pronounced secondary appendages. Altogether, the bioactive CsADM-Cl hydrogel established significant clinical potential to support wound healing as an apt injectable antibacterial matrix to encounter unmet challenges concerning critical burn wounds.
- Subjects :
- antioxidant
Polymers and Plastics
Angiogenesis
Acellular Dermal Matrix (ADM)
Burn
Bioengineering
Matrix (biology)
Biomaterials
Chitosan
Extracellular matrix
chemistry.chemical_compound
Materials Chemistry
Humans
Acellular Dermis
DHF-I
Collagen (Col)
Wound Healing
Chitosan (CTS)
integumentary system
Chemistry
Hydrogels
Adhesion
Extracellular Matrix
antibacterial
Covalent bond
Biophysics
Burns
Wound healing
Antibacterial activity
Subjects
Details
- ISSN :
- 15264602 and 15257797
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Biomacromolecules
- Accession number :
- edsair.doi.dedup.....2b13ce50cd01a9531d79b2f6fda7a353