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Pilose antler peptide attenuates LPS-induced inflammatory reaction
- Source :
- International journal of biological macromolecules. 108
- Publication Year :
- 2017
-
Abstract
- The present study was designed to study the effects of pilose antler peptide (PAP) on primary culture of nucleus pulposus cells in intervertebral disc. We demonstrated that PAP significantly inhibited lipopolysaccharides (LPS) induced over-production of inflammatory factors including interleukin-1β (IL-1β), tumor necrosis Factor-α (TNF-α) and interleukin-6 (IL-6) in nucleus pulposus cells. PAP also attenuated increase of malondialdehyde (MDA) and decrease of superoxide dismutase (SOD) induced by LPS challenge in a concentration-dependent manner. Moreover, the expression of the protein levels of mitogen-activated protein kinase (MAPK)/nuclear transcription factor-κB(NF-κB) were increased accompanying with the LPS challenge, which were significantly reversed after PAP treatment. Our results demonstrated the ability of PAP to antagonize LPS-mediated inflammation in primary culture of nucleus pulposus in intervertebral disc, suggesting a beneficial potential for its clinical application.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
Lipopolysaccharides
Cell Survival
Inflammation
Pharmacology
Biochemistry
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Structural Biology
Malondialdehyde
medicine
Animals
Protein kinase A
Molecular Biology
biology
Superoxide Dismutase
NF-kappa B
NF-κB
General Medicine
Rats
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
chemistry
biology.protein
Cytokines
Tumor necrosis factor alpha
medicine.symptom
Inflammation Mediators
Mitogen-Activated Protein Kinases
Peptides
Nucleus
030217 neurology & neurosurgery
Signal Transduction
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....2b114ff4cea59e5805414c036e4f7392