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The Effect of Long Term Caffeine Treatment on Hypoxic-Ischemic Brain Damage in the Neonate
- Source :
- Europe PubMed Central
- Publication Year :
- 1995
- Publisher :
- Springer Science and Business Media LLC, 1995.
-
Abstract
- There is considerable concern over the widespread use of caffeine during and after pregnancy. We have therefore examined the effect of perinatal caffeine use on the vulnerability of the immature brain to hypoxic ischemia (HI). Rat pups were exposed to caffeine during the first 7 d after birth by addition of a low or a high dose (0.3 or 0.8 g/L) of caffeine to the drinking water of their dams. At 7 d the pups were exposed to unilateral carotid occlusion+exposure to 7.70% oxygen for 100 min. The extent of HI brain damage was evaluated 2 wk after the insult. The effects of caffeine on A1 and A2a receptors, A1 mRNA and A2a mRNA, were examined by receptor autoradiography and in situ hybridization. Caffeine, theobromine, theophylline, and paraxanthine were analyzed in plasma of separate animals. Exposure to caffeine reduced HI brain damage from 40.3 +/- 3.2% in controls to 29.8 +/- 4.0% (p < 0.05) in low dose and 33.7 +/- 3.9% (NS) in the high dose group. The A1 receptor density measured as [3H]-1,3-dipropyl-8-cyclopentyl xanthine ([3H]-DPCPX) binding was not significantly affected after low dose caffeine but increased in the brain of rat pups in the high dose group. The A2a receptor density measured as [3H]-2[p-(2-carbonylethyl)-phenethylamino]-5'-N- ethylcarboxamidoadenosine ([3H]-CGS 21680) binding and the expression of A1 mRNA and A2a mRNA were not altered by caffeine treatment. In conclusion, low dose caffeine exposure (plasma levels corresponding to umbilical cord plasma in newborns of coffee-consuming mothers) reduced HI brain damage by 30% in 7-d-old rats.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- medicine.medical_specialty
Ischemia
Brain damage
Brain Ischemia
chemistry.chemical_compound
Adenosine A1 receptor
Pregnancy
Caffeine
Internal medicine
medicine
Animals
Theophylline
Rats, Wistar
Hypoxia, Brain
Theobromine
Paraxanthine
business.industry
Receptors, Purinergic P1
Brain
Xanthine
medicine.disease
Rats
Endocrinology
Animals, Newborn
chemistry
Maternal Exposure
Prenatal Exposure Delayed Effects
Xanthines
Pediatrics, Perinatology and Child Health
Central Nervous System Stimulants
Female
medicine.symptom
business
medicine.drug
Subjects
Details
- ISSN :
- 00313998
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Pediatric Research
- Accession number :
- edsair.doi.dedup.....2b0defd497807c2483df04a6d52164f4