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Exocytosis of catecholamine (CA)-containing and CA-free granules in chromaffin cells
- Source :
- Journal of Biological Chemistry
- Publication Year :
- 2001
-
Abstract
- Recent evidence suggests that endocytosis in neuroendocrine cells and neurons can be tightly coupled to exocytosis, allowing rapid retrieval from the plasma membrane of fused vesicles for future use. This can be a much faster mechanism for membrane recycling than classical clathrin-mediated endocytosis. During a fast exo-endocytotic cycle, the vesicle membrane does not fully collapse into the plasma membrane; nevertheless, it releases the vesicular contents through the fusion pore. Once the vesicle is depleted of transmitter, its membrane is recovered without renouncing its identity. In this report, we show that chromaffin cells contain catecholamine-free granules that retain their ability to fuse with the plasma membrane. These catecholamine-free granules represent 7% of the total population of fused vesicles, but they contributed to 47% of the fusion events when the cells were treated with reserpine for several hours. We propose that rat chromaffin granules that transiently fuse with the plasma membrane preserve their exocytotic machinery, allowing another round of exocytosis.
- Subjects :
- Vesicle fusion
Patch-Clamp Techniques
Vesicle
Chromaffin Cells
Endocytic cycle
Electric Conductivity
Cell Biology
Kiss-and-run fusion
Biology
Endocytosis
Biochemistry
Exocytosis
Cell biology
Rats
Rats, Sprague-Dawley
Membrane
Catecholamines
Animals
Chromaffin Granules
Patch clamp
Molecular Biology
Cells, Cultured
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....2b07ef93bb8934f360dbd47a2c99fa6c