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Molecular characterisation of a field strain of bubaline herpesvirus isolated from buffaloes (Bubalus bubalis ) after pharmacological reactivation
- Source :
- Scopus-Elsevier
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- Two healthy buffaloes (Bubalus bubalis) in a herd which had not been vaccinated against infectious bovine rhinotracheitis (IBR), were selected for their seropositivity for anti-bovine herpesvirus type 1 (BoHV-1) glycoprotein E antibodies, and injected intramuscularly daily with dexamethasone for five consecutive days (day 1 to day 5) to reactivate any latent herpesvirus. Blood samples and nasal and vaginal swabs were collected daily from day 5 to day 15 from each buffalo for virological examination. All the vaginal swabs and blood samples were negative, but 13 of the 22 nasal swabs were positive; a cytopathic effect was observed in primary cultures of bovine fetal lung cells, and the viral isolates were identified as a herpesvirus by PCR. The viral strains were characterised by the sequence analysis of the genes coding for glycoproteins D and B, and the gene sequences were then used for phylogenetic analysis. The isolates from both buffaloes appeared identical at the level of the two genes, and were more closely related to bovine herpesvirus type 5 than to BoHV-1.
- Subjects :
- Buffaloes
Sequence analysis
Molecular Sequence Data
Antibodies, Viral
Polymerase Chain Reaction
Dexamethasone
law.invention
law
Animals
Amino Acid Sequence
Infectious Bovine Rhinotracheitis
Phylogeny
Polymerase chain reaction
Herpesvirus 1, Bovine
Cytopathic effect
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
General Veterinary
biology
Viral Vaccine
Viral Vaccines
General Medicine
biology.organism_classification
Virology
Nasal Swab
DNA, Viral
Herd
biology.protein
Cattle
Bubalus
Antibody
Subjects
Details
- ISSN :
- 00424900
- Volume :
- 154
- Database :
- OpenAIRE
- Journal :
- Veterinary Record
- Accession number :
- edsair.doi.dedup.....2af7d4a903e022645c99f8cdcf002f7e