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Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2020
-
Abstract
- Made available in DSpace on 2021-06-25T10:44:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01 Preexisting/pregestational diabetes enhances the risk of birth defects. Several factors have been involved during the implantation process, such as cytokines (granulocyte-macrophage-colony-stimulating factor [GM-CSF]). The objective was to evaluate the effects of two levels of diabetes on the redox status of preimplantation embryos during the implantation process to comprehend how both are involved in embryo and fetal viability against maternal diabetes. Female Sprague-Dawley rats received streptozotocin at birth (mild diabetes [MD]) or at adulthood (severe diabetes [SD]) to obtain two experimental diabetes intensities. After confirming the diabetic status, the nondiabetic and diabetic groups were mated around day 110 of life. At gestational day (GD) 21, fetuses were assessed for viability and malformations and ovaries for embryo loss before implantation. Other pregnant nondiabetic and diabetic rats were sacrificed at GD2-4 for maternal and preimplantation embryo oxidative stress markers, maternal serum insulin, uterine fluid GM-CSF, and preimplantation embryo morphological analysis. MD and SD caused abnormal redox levels, lower GM-CSF and insulin levels during the preimplantation period, and embryonic loss before implantation. SD caused lower fetal viability and higher fetal malformation percentages at GD21. The SD dam-derived preimplantation embryos presented lower glutathione levels and higher thiobarbituric acid reactive substances concentration at GD3 and an increased frequency of abnormal preimplantation embryos at GD4. In conclusion, preexisting diabetes leads to complications in the implantation process. Furthermore, maternal oxidative stress and other metabolic changes alter the redox state and morphological structure of preimplantation embryos, contributing to damaged growth and development in late pregnancy. Laboratory of Experimental Research on Gynecology and Obstetrics Postgrad. Course on Tocogynecology Botucatu Med. Sch. S. Paulo State Univ. (Unesp) Botucatu S. Paulo State Laboratory of Experimental Research on Gynecology and Obstetrics Postgrad. Course on Tocogynecology Botucatu Med. Sch. S. Paulo State Univ. (Unesp) Botucatu S. Paulo State
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Embryonic Development
Biology
medicine.disease_cause
Congenital Abnormalities
Diabetes Mellitus, Experimental
Andrology
Rats, Sprague-Dawley
RATOS
03 medical and health sciences
0302 clinical medicine
Diabetes mellitus
medicine
Animals
oxidative stress
rat
Embryo Implantation
Fetus
030219 obstetrics & reproductive medicine
Fetal viability
Insulin
Embryo
Cell Biology
General Medicine
Streptozotocin
medicine.disease
Rats
pregestational
Oxidative Stress
fetus
030104 developmental biology
Reproductive Medicine
Gestation
Female
hyperglycemia
Oxidative stress
medicine.drug
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Accession number :
- edsair.doi.dedup.....2af610bafc2c74b79dbd8a0c21b63c6c