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Human muscle fiber type specific insulin signaling - Impact of obesity and type 2 diabetes
- Source :
- Albers, P H, Pedersen, A J T, Birk, J B, Kristensen, D E, Vind, B F, Baba, O, Nøhr, J, Højlund, K & Wojtaszewski, J F P 2015, ' Human muscle fiber type specific insulin signaling-Impact of obesity and type 2 diabetes ', Diabetes, vol. 64, no. 2, pp. 485-497 . https://doi.org/10.2337/db14-0590
- Publication Year :
- 2015
-
Abstract
- Skeletal muscle is a heterogeneous tissue composed of different fiber types. Studies suggest that insulin-mediated glucose metabolism is different between muscle fiber types. We hypothesized that differences are due to fiber type–specific expression/regulation of insulin signaling elements and/or metabolic enzymes. Pools of type I and II fibers were prepared from biopsies of the vastus lateralis muscles from lean, obese, and type 2 diabetic subjects before and after a hyperinsulinemic-euglycemic clamp. Type I fibers compared with type II fibers have higher protein levels of the insulin receptor, GLUT4, hexokinase II, glycogen synthase (GS), and pyruvate dehydrogenase-E1α (PDH-E1α) and a lower protein content of Akt2, TBC1 domain family member 4 (TBC1D4), and TBC1D1. In type I fibers compared with type II fibers, the phosphorylation response to insulin was similar (TBC1D4, TBC1D1, and GS) or decreased (Akt and PDH-E1α). Phosphorylation responses to insulin adjusted for protein level were not different between fiber types. Independently of fiber type, insulin signaling was similar (TBC1D1, GS, and PDH-E1α) or decreased (Akt and TBC1D4) in muscle from patients with type 2 diabetes compared with lean and obese subjects. We conclude that human type I muscle fibers compared with type II fibers have a higher glucose-handling capacity but a similar sensitivity for phosphoregulation by insulin.
- Subjects :
- Male
Muscle Fibers, Fast-Twitch/metabolism
medicine.medical_specialty
Insulin/metabolism
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Type 2 diabetes
Muscle Fibers, Slow-Twitch/metabolism
Internal medicine
Obesity/metabolism
Internal Medicine
medicine
Insulin
Humans
Obesity
Glycogen synthase
Protein kinase B
biology
Signal Transduction/physiology
TBC1D1
Skeletal muscle
Middle Aged
medicine.disease
Diabetes Mellitus, Type 2/metabolism
Insulin receptor
Endocrinology
medicine.anatomical_structure
Muscle Fibers, Slow-Twitch
Diabetes Mellitus, Type 2
Gene Expression Regulation
Muscle Fibers, Fast-Twitch
biology.protein
Female
GLUT4
Signal Transduction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Albers, P H, Pedersen, A J T, Birk, J B, Kristensen, D E, Vind, B F, Baba, O, Nøhr, J, Højlund, K & Wojtaszewski, J F P 2015, ' Human muscle fiber type specific insulin signaling-Impact of obesity and type 2 diabetes ', Diabetes, vol. 64, no. 2, pp. 485-497 . https://doi.org/10.2337/db14-0590
- Accession number :
- edsair.doi.dedup.....2af568639de2d58f2719fd4d386c6053