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Drosophila CAPS Is an Essential Gene that Regulates Dense-Core Vesicle Release and Synaptic Vesicle Fusion
- Source :
- Neuron. 31:421-437
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- Calcium-activated protein for secretion (CAPS) is proposed to play an essential role in Ca2+-regulated dense-core vesicle exocytosis in vertebrate neuroendocrine cells. Here we report the cloning, mutation, and characterization of the Drosophila ortholog (dCAPS). Null dCAPS mutants display locomotory deficits and complete embryonic lethality. The mutant NMJ reveals a 50% loss in evoked glutamatergic transmission, and an accumulation of synaptic vesicles at active zones. Importantly, dCAPS mutants display a highly specific 3-fold accumulation of dense-core vesicles in synaptic terminals, which was not observed in mutants that completely arrest synaptic vesicle exocytosis. Targeted transgenic CAPS expression in identified motoneurons fails to rescue dCAPS neurotransmission defects, demonstrating a cell nonautonomous role in synaptic vesicle fusion. We conclude that dCAPS is required for dense-core vesicle release and that a dCAPS-dependent mechanism modulates synaptic vesicle release at glutamatergic synapses.
- Subjects :
- Embryo, Nonmammalian
Vesicle fusion
Neuroscience(all)
Molecular Sequence Data
Neuromuscular Junction
Vesicular Transport Proteins
Glutamic Acid
Motor Activity
Neurotransmission
Biology
Membrane Fusion
Synaptic Transmission
Synaptic vesicle
Exocytosis
Animals, Genetically Modified
03 medical and health sciences
0302 clinical medicine
Animals
Amino Acid Sequence
Cloning, Molecular
Conserved Sequence
030304 developmental biology
Motor Neurons
0303 health sciences
Genes, Essential
Sequence Homology, Amino Acid
General Neuroscience
Calcium-Binding Proteins
SNAP25
Munc-18
Kiss-and-run fusion
Rats
Cell biology
Synaptic vesicle exocytosis
Drosophila melanogaster
CADPS2
Synaptic Vesicles
Sequence Alignment
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 08966273
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Neuron
- Accession number :
- edsair.doi.dedup.....2af4f8dff041bd64db4d8a4da7152508