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Bioprinting of high cell-density constructs leads to controlled lumen formation with self-assembly of endothelial cells
- Source :
- Journal of tissue engineering and regenerative medicineREFERENCES. 13(10)
- Publication Year :
- 2018
-
Abstract
- Active nutrient supply and waste product removal are key requirements for the fabrication of long-term viable and functional tissue constructs of considerable size. This work aims to contribute to the fabrication of artificial perfusable networks with a bioprinting process, based on drop-on-demand (DoD) printing of primary endothelial cell (EC) suspension bioink (25 × 106 ± 3 × 106 cells/ml). The process results in prescribed lumen between two hydrogel layers, allowing its integration in common layering based bioprinting processes. Low volume bioink droplets (appr. 10 nl) as building blocks were deposited between two fibrin or Collagen I layers to realize shapeable, cell-rich aggregates. Unattainable with manual positioning, DoD printing allowed precise fabrication of various designs, such as spheroidal-, line-shaped, and Y-branch cellular structures, with a mean lateral extension of 285 ± 81 μm. For basic characterization, the cell suspension building blocks were systematically compared with preformed spheroids of the same cell type, passage, and number. Post printing investigations of initially loose cell arrangements showed self-assembly and formation of central lumen with a mean cross-sectional area of Olumen = 6,400 μm2 at Day 3, lined by a single layer of CD31 positive ECs, as evaluated by confocal microscopy. Originating from this main lumen smaller, undirected side branches (Obranches = 740 μm2 ) were formed by sprouting cells, inducing a first step towards a simplistic hierarchically organized network. These lumen could prospectively help for tissue construct perfusion in vitro or, potentially, as niche for angiogenesis of host vascularization in implants.
- Subjects :
- Adult
Cell type
Fabrication
Materials science
Angiogenesis
0206 medical engineering
Biomedical Engineering
Medicine (miscellaneous)
Lumen (anatomy)
Cell Count
02 engineering and technology
Regenerative Medicine
law.invention
Biomaterials
03 medical and health sciences
Confocal microscopy
law
Spheroids, Cellular
Human Umbilical Vein Endothelial Cells
Humans
Regeneration
Process (anatomy)
030304 developmental biology
Cell Aggregation
0303 health sciences
Tissue Scaffolds
Spheroid
Bioprinting
020601 biomedical engineering
Endothelial stem cell
Ink
Biomedical engineering
Subjects
Details
- ISSN :
- 19327005
- Volume :
- 13
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of tissue engineering and regenerative medicineREFERENCES
- Accession number :
- edsair.doi.dedup.....2aecef68401a6759a9a5c912f9f4fc2c