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Colorimetric paper-based immunosensor for simultaneous determination of fetuin B and clusterin toward early Alzheimer’s diagnosis
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2019
-
Abstract
- Alzheimer's disease is a devastating condition characterized by a progressive and slow brain decay in elders. Here, we developed a paper-based lateral flow immunoassay for simultaneous and fast determination of Alzheimer's blood biomarkers, fetuin B and clusterin. Selective antibodies to targeted biomarkers were immobilized on gold nanoparticles (AuNPs) and deposited on paper pads. After adding the sample on the paper-based device, the biofluid laterally flows toward the selective antibody, permitting AuNP-Ab accumulation on the test zone, which causes a color change from white to pink. Image analysis was performed using a customized algorithm for the automatic recognition of the area of analysis and color clustering. Colorimetric detection was compared to electrochemical methods for the precise quantification of biomarkers. The best performance was found for the color parameter "L*". Good linearity (R2 = 0.988 and 0.998) and reproducibility (%RSD = 2.79% and 1.82%, N = 3) were demonstrated for the quantification of fetuin B and clusterin, respectively. Furthermore, the specificity of the immunosensor was tested on mixtures of proteins, showing negligible cross-reactivity and good performance in complex environments. We believe that our biosensor has a potential for early-stage diagnosis of Alzheimer's disease and toward a better understanding of Alzheimer's developing mechanisms.
- Subjects :
- Paper
General Physics and Astronomy
Metal Nanoparticles
02 engineering and technology
Biosensing Techniques
010402 general chemistry
01 natural sciences
BIOMARCADORES
Alzheimer Disease
Humans
General Materials Science
Fetuin b
Colorimetry
Immunoassay
Reproducibility
Chromatography
Clusterin
biology
Chemistry
General Engineering
Paper based
021001 nanoscience & nanotechnology
Fetuin-B
0104 chemical sciences
Colloidal gold
biology.protein
Gold
0210 nano-technology
Biosensor
Lateral flow immunoassay
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Accession number :
- edsair.doi.dedup.....2ad9efd534bba32e1754bc1d480238d9