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A role for primary cilia in glutamatergic synaptic integration of adult-born neurons
- Source :
- Cilia, Cilia, Vol 1, Iss Suppl 1, p P31 (2012)
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- New neurons are continually born throughout adulthood in the subventricular zone of the lateral ventricle and in the subgranular zone of the dentate gyrus in the hippocampus. The sequential synaptic integration of adult-born neurons has been widely examined in rodents, but the mechanisms regulating the integration remain largely unknown. The primary cilium, a microtubule–based signaling center, plays essential roles in vertebrate development, including the development of the central nervous system. We examined the assembly and function of the primary cilium in the synaptic integration of adult-born hippocampal neurons. Strikingly, primary cilia are absent in young adult-born neurons but assemble precisely at the stage when newborn neurons approach their final destination, further extend dendrites and form synapses with entorhinal cortical projections. Conditional cilia deletion from adult-born neurons induced severe defects in dendritic refinement and synapse formation. Primary cilia deletion leads to enhanced Wnt/beta-catenin signaling which may account for these developmental defects. Taken together, our study identifies the assembly of primary cilia as a critical regulatory event in the dendritic refinement and synaptic integration of adult-born neurons.
- Subjects :
- Male
Patch-Clamp Techniques
Time Factors
Kinesins
Hippocampus
Channelrhodopsin
Hippocampal formation
Subgranular zone
Mice
0302 clinical medicine
Ganglia, Spinal
RNA, Small Interfering
beta Catenin
6-Cyano-7-nitroquinoxaline-2,3-dione
Neurons
0303 health sciences
Microscopy, Confocal
lcsh:Cytology
General Neuroscience
Cilium
Wnt signaling pathway
Cell biology
medicine.anatomical_structure
Female
Plant Lectins
Adenylyl Cyclases
Signal Transduction
Neurogenesis
Transgene
Glutamic Acid
Subventricular zone
Mice, Transgenic
Biology
Transfection
03 medical and health sciences
Glutamatergic
Channelrhodopsins
Microtubule
medicine
Animals
Cilia
Patch clamp
lcsh:QH573-671
030304 developmental biology
Analysis of Variance
Dentate gyrus
Calcium-Binding Proteins
Excitatory Postsynaptic Potentials
Dendrites
Cell Biology
beta-Galactosidase
nervous system
Synapses
Poster Presentation
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15461726 and 10976256
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Nature Neuroscience
- Accession number :
- edsair.doi.dedup.....2ad20a2cac8fc95c5c2f251d8744bb99