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Local potentiation of stress-responsive genes by upstream noncoding transcription
- Source :
- Nucleic Acids Research
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- It has been postulated that a myriad of long noncoding RNAs (lncRNAs) contribute to gene regulation. In fission yeast, glucose starvation triggers lncRNA transcription across promoter regions of stress-responsive genes including fbp1 (fructose-1,6-bisphosphatase1). At the fbp1 promoter, this transcription promotes chromatin remodeling and fbp1 mRNA expression. Here, we demonstrate that such upstream noncoding transcription facilitates promoter association of the stress-responsive transcriptional activator Atf1 at the sites of transcription, leading to activation of the downstream stress genes. Genome-wide analyses revealed that ∼50 Atf1-binding sites show marked decrease in Atf1 occupancy when cells are treated with a transcription inhibitor. Most of these transcription-enhanced Atf1-binding sites are associated with stress-dependent induction of the adjacent mRNAs or lncRNAs, as observed in fbp1. These Atf1-binding sites exhibit low Atf1 occupancy and high histone density in glucose-rich conditions, and undergo dramatic changes in chromatin status after glucose depletion: enhanced Atf1 binding, histone eviction, and histone H3 acetylation. We also found that upstream transcripts bind to the Groucho-Tup1 type transcriptional corepressors Tup11 and Tup12, and locally antagonize their repressive functions on Atf1 binding. These results reveal a new mechanism in which upstream noncoding transcription locally magnifies the specific activation of stress-inducible genes via counteraction of corepressors.
- Subjects :
- 0301 basic medicine
Chromatin Immunoprecipitation
RNA, Untranslated
Saccharomyces cerevisiae Proteins
Transcription, Genetic
Response element
Saccharomyces cerevisiae
Transcription coregulator
Regulatory Sequences, Nucleic Acid
Biology
Histones
03 medical and health sciences
Upstream activating sequence
0302 clinical medicine
Stress, Physiological
Gene Expression Regulation, Fungal
Genetics
General transcription factor
Gene regulation, Chromatin and Epigenetics
Pioneer factor
High-Throughput Nucleotide Sequencing
Acetylation
Promoter
Chromatin Assembly and Disassembly
Molecular biology
Glucose
030104 developmental biology
Gene Expression Regulation
Transcription Coactivator
Chromatin immunoprecipitation
030217 neurology & neurosurgery
Protein Binding
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....2acb12df48ffeb9cee04e5b862fdb462