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Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 8 (2021), PLoS ONE, Vol 16, Iss 8, p e0253574 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science, 2021.
-
Abstract
- Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the ongoing coronavirus disease (COVID-19) pandemic which is characterized by respiratory illness and severe pneumonia, and currently accounts for > 2.5 million deaths worldwide. Recently, diverse mutations in the spike protein of SARS-CoV-2 were reported in United Kingdom (Alpha) and South Africa (Beta) strains which raise concerns over the potential increase in binding affinity towards the host cell receptor and diminished host neutralization capabilities. In order to study the effect of mutation in the binding efficiency of SARS-CoV-2 receptor binding domain (RBD) with anti-SARS-CoV/CoV-2 monoclonal antibodies (mAbs), we have produced SARS-CoV-2 RBD and two variants SARS-CoV-2 RBD (Alpha RBD and Beta RBD) in Nicotiana benthamiana by transient expression. Plant-produced SARS-CoV-2 RBD-Fc, Alpha RBD-Fc and Beta RBD-Fc exhibited specific binding to human angiotensin converting enzyme 2 (ACE2) receptor determined by ELISA. Intriguingly, the binding of plant-produced SARS-CoV-2 RBD proteins to plant-produced mAbs CR3022, B38, and H4 was found to be different depending on the variant mutation. In contrary to the plant-produced SARS-CoV-2 RBD-Fc and Alpha RBD-Fc, Beta RBD-Fc variant showed weak binding affinity towards the mAbs. The result suggested that the Beta RBD variant might have acquired partial resistance to neutralizing antibodies compared to other variants. However, further studies with sera from convalescent or vaccinated individuals are required to confirm this finding.
- Subjects :
- RNA viruses
Viral Diseases
Coronaviruses
Physiology
viruses
Nicotiana benthamiana
Plasma protein binding
medicine.disease_cause
Biochemistry
Antigen-Antibody Reactions
Medical Conditions
Immune Physiology
Enzyme-Linked Immunoassays
Receptor
Pathology and laboratory medicine
Flowering Plants
Coronavirus
Vaccines
Multidisciplinary
Immune System Proteins
biology
Microbial Mutation
virus diseases
Eukaryota
Antibodies, Monoclonal
Medical microbiology
Plants
Recombinant Proteins
Infectious Diseases
Viruses
Spike Glycoprotein, Coronavirus
Medicine
Angiotensin-Converting Enzyme 2
Antibody
SARS CoV 2
Pathogens
Research Article
Protein Binding
Nicotiana
Substitution Mutation
SARS coronavirus
Infectious Disease Control
medicine.drug_class
Science
Immunology
Alpha (ethology)
Monoclonal antibody
Research and Analysis Methods
Microbiology
Antibodies
Protein Domains
Virology
Tobacco
medicine
Genetics
Humans
Immunoassays
Medicine and health sciences
Biology and life sciences
SARS-CoV-2
Point mutation
fungi
Organisms
Viral pathogens
Proteins
COVID-19
Covid 19
Viral Vaccines
biology.organism_classification
respiratory tract diseases
Microbial pathogens
Mutation
biology.protein
Immunologic Techniques
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....2ac21a0472b2c864f01c0e213b919ac9