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Protein-responsive rolling circle amplification as a tandem template to drive amplified transduction of fluorescence signal probes for highly sensitive immunoassay
- Source :
- Chemical Communications. 54:10195-10198
- Publication Year :
- 2018
- Publisher :
- Royal Society of Chemistry (RSC), 2018.
-
Abstract
- Proximity binding-induced DNA assemblies possessing outstanding flexibility and diversity can provide great accessibility for output translation of amplifiable DNA probes or directly detectable signals. Based on a novel protein-responsive DNA assembly constructed via proximity binding-initiated rolling circle amplification (RCA), we present a sensitive and specific fluorescence resonance energy transfer (FRET) strategy for the immunoassay of mucin 1 (MUC1). The resultant RCA product containing numerous and continuous repeats as the tandem template accelerates the transduction of signal probes through the disassembly of triple-helix stem structure (THSS), achieving a significantly amplified FRET signal readout.
- Subjects :
- Biotin
02 engineering and technology
010402 general chemistry
01 natural sciences
Antibodies
Fluorescence
Catalysis
chemistry.chemical_compound
Transduction (genetics)
Limit of Detection
Cell Line, Tumor
Fluorescence Resonance Energy Transfer
Materials Chemistry
medicine
Humans
Fluorescent Dyes
Immunoassay
medicine.diagnostic_test
Tandem
Rhodamines
Hybridization probe
Mucin-1
Metals and Alloys
Nucleic Acid Hybridization
DNA
General Chemistry
Fluoresceins
021001 nanoscience & nanotechnology
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Förster resonance energy transfer
chemistry
Rolling circle replication
Ceramics and Composites
Biophysics
0210 nano-technology
Nucleic Acid Amplification Techniques
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi.dedup.....2ab659725c991ce61cb2520aa3bee34d