Brief Note Mendelian inheritance, linkage and genotypic disequilibrium in microsatellite loci isolated from Hymenaea courbaril (Leguminosae)

Made available in DSpace on 2013-09-27T14:53:04Z (GMT). No. of bitstreams: 1 WOS000308817800014.pdf: 379718 bytes, checksum: a957238b7822bf2ada55c2c4a88d949f (MD5) Previous issue date: 2012-01-01 Made available in DSpace on 2013-09-30T19:24:50Z (GMT). No. of bitstreams: 1 WOS000308817800014.pdf: 379718 bytes, checksum: a957238b7822bf2ada55c2c4a88d949f (MD5) Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:34:50Z No. of bitstreams: 1 WOS000308817800014.pdf: 379718 bytes, checksum: a957238b7822bf2ada55c2c4a88d949f (MD5) Made available in DSpace on 2014-05-20T15:34:50Z (GMT). No. of bitstreams: 1 WOS000308817800014.pdf: 379718 bytes, checksum: a957238b7822bf2ada55c2c4a88d949f (MD5) Previous issue date: 2012-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) European Commission (SEEDSOURCE Project) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) The Neotropical tree Hymenaea courbaril, locally known as Jatoba, is a valuable source of lumber and also produces comestible and medicinal fruit. We characterized Mendelian inheritance, linkage and genotypic disequilibrium at nine microsatellite loci isolated from H. courbaril, in order to determine if they would provide accurate estimates of population genetic parameters of this important Amazon species. The study was made on 250 open-pollinated offspring originated from 14 seed trees. Only one of nine loci presented significant deviation from the expected Mendelian segregation (1: 1). Genotypic disequilibrium between pairwise loci was investigated based on samples from 55 adult and 56 juvenile trees. No genetic linkage between any paired loci was observed. After Bonferroni's corrections for multiple tests, we found no evidence of genotypic disequilibrium between pairs of loci. We conclude that this set of loci can be used for genetic diversity/structure, mating system, gene flow, and parentage analyses in H. courbaril populations. Inst Florestal São Paulo, São Paulo, Brazil Inst Pesquisas Jardim Bot Rio de Janeiro, Inst Pesquisas, Rio de Janeiro, RJ, Brazil Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Amazonia Oriental, Belem, Para, Brazil Univ Estadual São Paulo Julio de Mesquita Filho, Fac Engn Ilha Solteira, Ilha Solteira, SP, Brazil Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Florestas, Curitiba, Parana, Brazil Inst Nacl de Pesquisas da Amazonia, Lab Genet & Biol Reprodut Plantas LARGEN, Manaus, Amazonas, Brazil Univ Estadual São Paulo Julio de Mesquita Filho, Fac Engn Ilha Solteira, Ilha Solteira, SP, Brazil European Commission (SEEDSOURCE Project): 003708 CNPq: 2006/04490-9