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P130cas is required for TGF-β1-mediated epithelial-mesenchymal transition in lung cancer
- Source :
- Oncology Letters
- Publication Year :
- 2013
-
Abstract
- In lung cancer A549 cells, the present study evaluated the associations between p130cas expression and the activation of p38 or Smad2, which are components of two of the main signaling pathways of transforming growth factor-β1 (TGF-β1), i.e., epithelial-mesenchymal transition (EMT) and apoptosis, respectively. TGF-β1-induced EMT was investigated by inspecting cell shape and cell migration, and by testing E-Cadherin, N-Cadherin and Vimentin biomarkers in p130cas-RNA interference (RNAi)-A549 cells. The changes in TGF-β1-induced apoptosis, i.e., cleaved Caspase-3 levels, were additionally analyzed following p130cas-RNAi. p130cas-knockdown decreased the phosphorylated (p)-p38 expression level, and blockaded the TGF-β1-induced activation of p-p38 in the A549 cells. p130cas-knockdown arrested cell migration and impaired TGF-β1-induced EMT in the A549 cells, characterized by changes in cell morphology and biomarker levels. However, p130cas-knockdown had no impact on the activation of Smad2 and the cleavage of Caspase-3. These results indicate that p130cas is a novel molecular ‘rheostat’ that alters the function of the TGF-β1 signaling pathway from tumor suppression to tumor promotion in lung cancer cells. The underlying mechanism warrants further study.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Cell
epithelial-mesenchymal transition
p38
Vimentin
TGF-β1
medicine
Epithelial–mesenchymal transition
non-small cell lung cancer
A549 cell
biology
business.industry
P130cas
Cell migration
Articles
Cell cycle
medicine.anatomical_structure
Oncology
breast cancer anti-estrogen resistance protein 1
biology.protein
Cancer research
Tumor promotion
Signal transduction
business
Corrigendum
Subjects
Details
- ISSN :
- 17921074
- Volume :
- 8
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Oncology letters
- Accession number :
- edsair.doi.dedup.....2a8f8176348cd9756631fa5bf6e0f6a6