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IN VIVO INCORPORATION OF l-[14C]SERINE INTO PHOSPHOLIPIDS AND PROTEINS OF THE SUBCELLULAR FRACTIONS OF DEVELOPING RAT BRAIN
- Source :
- Journal of Neurochemistry. 13:1189-1196
- Publication Year :
- 1966
- Publisher :
- Wiley, 1966.
-
Abstract
- SUMMARY A study was conducted on the in vivo incorporation of l-[14C]-serine into the lipids and proteins of the various subcellular fractions of the developing rat brain before and during the stage of active myelination. The total radioactivity in the various fractions at 12 days of age was higher than that at 3 days, while the radioactive specific activity was reversed. The specific activities of the proteins and lipids were higher at 3 days of age with the exception of the subcellular fraction containing myelin. At both ages the lipids of the various cellular fractions had similar specific activities, a finding that suggests a common source for lipid biosynthesis. Incorporation of radioactivity into the various phospholipids was in the following order: phosphatidyl serine > phosphatidyl ethanolamine > phosphatidal serine > sphingomyelin and phosphatidyl choline. Of all the phospholipids, the plasmalogens increased most in total radioactivity during the period when meylination was most active. Serine-containing phospholipids appear to be most tightly bound to proteins. The brain mitochrondrial fraction contained most of the phosphatidyl serine decarboxylase activity with some activity in the nuclei. Biosynthesis of phosphatdyil ethanolamine through decarboxylation of phosphatidyl serine could take place in rat brain. Four unidentified radioactive metabolites were found in the acid-soluble fraction in addition to l-[14C]serine.
- Subjects :
- Chromatography, Paper
Brain
Nerve Tissue Proteins
Mitochondrion
Biology
Biochemistry
Mitochondria
Rats
Serine
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Ethanolamine
Biosynthesis
chemistry
In vivo
Lipid biosynthesis
Animals
Autoradiography
lipids (amino acids, peptides, and proteins)
Specific activity
Sphingomyelin
Phospholipids
Subjects
Details
- ISSN :
- 14714159 and 00223042
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Neurochemistry
- Accession number :
- edsair.doi.dedup.....2a848ed407290274ddba1edfc167e305