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Different characteristics of chronic dibutyltin dichloride-induced pancreatitis and cholangitis in mouse and rat
- Source :
- Hepatobiliary & Pancreatic Diseases International. 19:169-174
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Background Current animal models of chronic pancreatitis (CP) often provide only limited pathophysiological insights since they incompletely reflect the human disease. CP induced by injection of dibutyltin dichloride (DBTC-pancreatitis) shares with human CP the important feature of extended fibrosis and would be an even more attractive model if it could be transferred from rats to mice, as recently suggested in the context of combined ethanol and DBTC application. This study aimed to evaluate the effects of DBTC in pancreas and liver of C57BL/6 mice, a strain commonly used to engineer genetic mouse models. Methods C57BL/6 mice and Lewis rats were exposed to variable doses of DBTC. After an investigation period of up to 4 weeks, laboratory findings and histopathological changes of pancreas and liver were evaluated. Results Chronic DBTC-pancreatitis in rats was characterized by acinar cell damage, ductal changes, fibrosis, and inflammatory cell infiltrates. Mice treated with DBTC at 6–8 mg/kg body weight, the standard doses in rats, showed transient increases of lipase activities but no morphological signs of chronic DBTC-pancreatitis 4 weeks after injection of the drug. Increased doses of 10–12 mg/kg DBTC were intolerable due to their high toxicity. In contrast, mice and rats presented with a similar histopathology of the liver that can be characterized as a chronic-proliferative DBTC-cholangitis with predominating damage and proliferation of the small bile ducts as well as secondary portal inflammatory cell infiltrates and a beginning portal fibrosis. Conclusions The DBTC-model cannot be transferred from rats to C57BL/6 mice with respect to chronic DBTC-pancreatitis, but might be of interest to study DBTC-cholangitis in both species.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Cholangitis
Context (language use)
Mice
03 medical and health sciences
0302 clinical medicine
Fibrosis
Organotin Compounds
Acinar cell
Animals
Medicine
Pancreas
Hepatology
business.industry
Gastroenterology
Lipase
medicine.disease
Rats
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Liver
Pancreatitis
Rats, Inbred Lew
030220 oncology & carcinogenesis
Portal fibrosis
Chronic Disease
Toxicity
030211 gastroenterology & hepatology
Histopathology
business
Subjects
Details
- ISSN :
- 14993872
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Hepatobiliary & Pancreatic Diseases International
- Accession number :
- edsair.doi.dedup.....2a803f751ab93b431dd5d784ee013702