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A RIKILT yeast estrogen bioassay (REA) for estrogen residue detection in urine of calves experimentally treated with 17ß-estradiol
- Source :
- Food Additives and Contaminants, 27(1), 19-28, Food Additives and Contaminants 27 (2010) 1
- Publication Year :
- 2010
-
Abstract
- 17beta-Estradiol is one of the most powerful sex steroids illegally used in bovine production. The objective of this study was to evaluate the application and the specificity of the RIKILT yeast estrogen bioassay (REA) for the detection of molecules with estrogenic activities in the urine of calves experimentally treated with anabolics. Four groups of six calves each received an injection of 17beta-estradiol intramuscularly (group B), androsterone and gliburide (group A), and testosterone (group C) molecules at different dosage for 40 days. Group D was the control. The ability of the REA test to detect estrogenic activity in urine samples from all animals was assessed. All estrogen-treated animals (group B) showed as being positive up to 7 days after administration of the highest dosage of 17beta-estradiol, while the other three groups showed as being negative. The identity of estrogenic molecules in the urine of group B (17beta-estradiol, 17alpha-estradiol) was confirmed by gas chromatography-mass spectrometry (GC/MS). This is the first time the REA test has been applied to detect 17beta-estradiol in the urine of calves treated with the hormone in vivo. The technique may offer an advantageous laboratory method for the veterinary surveillance of illegal steroid use.
- Subjects :
- Male
green fluorescent protein
Health, Toxicology and Mutagenesis
RIKILT - Business Unit Veiligheid & Gezondheid
Urine
Toxicology
chemistry.chemical_compound
Anabolic Agents
Genes, Reporter
beta-agonists
liquid-chromatography
Bioassay
tandem mass-spectrometry
anabolic-steroids
Testosterone
media_common
Androsterone
Estradiol
General Medicine
Recombinant Proteins
Biological Assay
hormones, hormone substitutes, and hormone antagonists
medicine.medical_specialty
Meat
bovine urine
receptor-alpha
medicine.drug_class
Green Fluorescent Proteins
Food Contamination
gc-ms
Saccharomyces cerevisiae
Biology
Gas Chromatography-Mass Spectrometry
Internal medicine
Prohibitins
medicine
media_common.cataloged_instance
Animals
Humans
European Union
European union
Public Health, Environmental and Occupational Health
Estrogen Receptor alpha
muscle-tissues
Estrogens
General Chemistry
17beta estradiol
growth promoters
Endocrinology
chemistry
Estrogen
RIKILT - Business Unit Safety & Health
Cattle
Food Science
Hormone
Subjects
Details
- Language :
- English
- ISSN :
- 0265203X
- Database :
- OpenAIRE
- Journal :
- Food Additives and Contaminants, 27(1), 19-28, Food Additives and Contaminants 27 (2010) 1
- Accession number :
- edsair.doi.dedup.....2a7f1f961e37be2b2c68c77f8acb5a90