Back to Search
Start Over
Polarised expression pattern of focal contact proteins in highly motile antigen presenting dendritic cells
- Source :
- Journal of Cell Science. 112:1685-1696
- Publication Year :
- 1999
- Publisher :
- The Company of Biologists, 1999.
-
Abstract
- Dendritic cells are professional antigen presenting cells that capture antigens and migrate to lymphoid tissues to elicit specific T cell responses. Here we used an in vitro differentiation system for generating highly motile dendritic cells from chicken bone marrow progenitors by employing the conditional v-Rel estrogen receptor (ER) fusion protein v-RelER. Molecular mechanisms of dendritic cell motility were investigated. Differentiation of v-relER progenitors into dendritic cells is associated with a reduction in cell-cell and cell-extracellular matrix interactions as cells acquire motility. We demonstrate that v-relER progenitors and dendritic cells express several adhesion receptors and components of adhesion complexes. Differentiation of v-relER cells was accompanied by downregulation of focal adhesion kinase (FAK), a key molecule of adhesion complexes, but ectopic FAK expression did not affect cell adhesion and motility. Interestingly, v-relER dendritic cells exhibit a polarised expression pattern of actin and vimentin, with actin being highly concentrated at the leading edge of the cells where lamellipodia are formed. FAK, paxillin and tyrosine phosphorylated proteins are found at both poles of the cell and colocalise with actin at the leading edge, while surface beta1 integrin is confined to the uropod at the rear. CD34(+)stem cell-derived human dendritic cells also exhibited an elongated bipolar morphology, mode of migration and a polarised pattern of actin-vimentin expression similar to v-relER dendritic cells.
- Subjects :
- Oncogene Proteins v-rel
CD40
biology
Follicular dendritic cells
Podosome
Recombinant Fusion Proteins
Retroviridae Proteins, Oncogenic
Cell Differentiation
Cell Communication
Chick Embryo
Dendritic Cells
Cell Biology
Dendritic cell
Cell biology
Focal adhesion
Receptors, Estrogen
biology.protein
Animals
Humans
Antigen-presenting cell
Cell adhesion
Cell Adhesion Molecules
Chickens
Paxillin
Subjects
Details
- ISSN :
- 14779137 and 00219533
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Science
- Accession number :
- edsair.doi.dedup.....2a7eb4c8dbbac101b056b90f3fc0df56