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Accumulation of Advanced Glycation Endproducts and Subclinical Inflammation in Deep Tissues of Adult Patients With and Without Diabetes
- Source :
- Canadian journal of diabetes. 42(5)
- Publication Year :
- 2017
-
Abstract
- Objectives Advanced glycation endproducts (AGEs) play a key role in the development of foot complications in people with diabetes. Skin autofluorescence (AF) might noninvasively determine tissue accumulation of AGEs. This study evaluated the association between skin AF and AGE contents in the deep tissues of those with diabetes and the further consequences of such contents. Methods Between September 2014 and September 2015, we studied 33 patients, with and without diabetes, who had received lower-limb amputations. Skin AF was measured. Artery, nerve and skin were harvested during surgery. AGE contents were quantified using high-performance liquid chromatography mass spectrometry and were located by immunohistochemistry staining. Inflammatory cells were also located by immunohistochemistry, immunofluorescence and scanning electron microscopy. Results Values of skin AF and AGE contents in artery, nerve and skin in patients with diabetes were higher than those in healthy patients. Skin AF was strongly affected by AGE contents in these tissues. AGE contents in various tissues were strongly correlated with each other. Differing AGEs were deposited in similar manners in the same tissues and were accompanied by inflammatory cells. Conclusions AGE contents were strongly correlated with each other and were accompanied by inflammatory cells. Skin AF measurement could provide information about the systemic accumulation of AGEs.
- Subjects :
- 0301 basic medicine
Adult
Glycation End Products, Advanced
Male
Pathology
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
030209 endocrinology & metabolism
Immunofluorescence
03 medical and health sciences
0302 clinical medicine
Endocrinology
Nerve Fibers
Diabetes mellitus
Internal Medicine
medicine
Diabetes Mellitus
Humans
Subclinical inflammation
Skin
Inflammation
integumentary system
Adult patients
medicine.diagnostic_test
business.industry
Tissue Extracts
Optical Imaging
General Medicine
Arteries
medicine.disease
Advanced Glycation Endproducts
Staining
030104 developmental biology
medicine.anatomical_structure
Case-Control Studies
Asymptomatic Diseases
Immunohistochemistry
Female
business
Artery
Subjects
Details
- ISSN :
- 23523840
- Volume :
- 42
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Canadian journal of diabetes
- Accession number :
- edsair.doi.dedup.....2a73a4abccb36d6c0bae599a11990bd1