Cloning and characterisation of a bovine P2Y receptor

Using a chick P2Y1 receptor cDNA probe we have isolated a mammalian P2Y receptor clone from a bovine aortic endothelial cell Library. The sequence has a high degree of similarity to the chick P2Y1 clone. When transfected into the Jurkat cell line, the cDNA conferred sensitivity to purinoceptor agonists. Using fura-2 loaded cells the potency order at the receptor was found to be 2-methlythioadenosine 5′ triphosphate = adenosine 5′ diphosphate > adenosine 5′ triphosphate >> α,β-methyleneadenosine 5′ triphosphate and uridine 5′ triphosphate. This corresponds to the agonist potency order expected for the bovine aortic endothelial cell P2Y receptor.