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Oncoproteins and proliferation markers in synovial sarcomas: a clinicopathologic study of 19 cases
- Source :
- Journal of Cancer Research and Clinical Oncology. 128:610-616
- Publication Year :
- 2002
- Publisher :
- Springer Science and Business Media LLC, 2002.
-
Abstract
- Purpose. The objective of this study was to evaluate synovial sarcomas for the expression of oncogenic proteins (Her2/neu, EGFR, Bcl-2, p53) and proliferation markers (Ki-67, Topoisomerase 2α), as possible markers of prognostic significance. Methods. From 17 patients with synovial sarcomas 19 tumors (15 primary, 2 recurrent, and 2 metastatic) were selected on the basis of characteristic histology, the expression of at least one epithelial marker, and/or the presence of t(X;18). Adequate follow-up was available in all cases. Results. The tumors were tested immunohistochemically and were found to express multiple oncogenic proteins. Four of 19 synovial sarcomas (21%) demonstrated nuclear over-expression of p53 protein; 18 of 19 tumors (94%) stained positive for Bcl-2; and 13 of 19 tumors (68%) were immunoreactive with EGFR. Of particular interest was the frequent expression of Her2/neu, an oncogenic protein more commonly observed in epithelial neoplasms. Ten of 19 tumors (52%, 7 monophasic and 3 biphasic) showed positive cytoplasmic and membranous staining with Her2/neu (HercepTest, DAKO). The staining intensity ranged from 1+ to 2+. Cellular expression of Her2/neu was independent of EGFR positivity and showed no association with proliferative activity of the tumors. FISH analysis of eight positive cases showed no evidence of Her2/neu gene amplification. Among the non-metastatic tumors, we found a significant correlation between Ki-67 and Topoisomerase 2α. Spearman's correlation co-efficient was 0.86 with P=0.001 (n=17). Conclusions. In this relatively small series of cases, we found no definite correlation between the over-expression of Her2/neu and clinical outcome. The over-expression of p53 was significantly associated with clinical outcome (Fisher's exact test, P=0.02).
- Subjects :
- Adult
Male
Cancer Research
medicine.medical_specialty
Pathology
Receptor, ErbB-2
Soft Tissue Neoplasms
HercepTest
HER2/neu
Immunoenzyme Techniques
Sarcoma, Synovial
Antigens, Neoplasm
Internal medicine
Biomarkers, Tumor
medicine
Humans
Neoplasm
In Situ Hybridization, Fluorescence
Aged
Hematology
biology
Histology
General Medicine
Middle Aged
Prognosis
medicine.disease
Synovial sarcoma
DNA-Binding Proteins
ErbB Receptors
Gene Expression Regulation, Neoplastic
Exact test
DNA Topoisomerases, Type II
Ki-67 Antigen
Proto-Oncogene Proteins c-bcl-2
Oncology
biology.protein
Female
Sarcoma
Tumor Suppressor Protein p53
Follow-Up Studies
Subjects
Details
- ISSN :
- 01715216
- Volume :
- 128
- Database :
- OpenAIRE
- Journal :
- Journal of Cancer Research and Clinical Oncology
- Accession number :
- edsair.doi.dedup.....2a6842d073db9a86ed51f8921b1fe908