Back to Search
Start Over
Neurite outgrowth resistance to rho kinase inhibitors in PC12 Adh cell
- Source :
- Cell biology international. 39(5)
- Publication Year :
- 2014
-
Abstract
- Rho kinase (ROCK) inhibitor is a promising agent for neural injury disorders, which mechanism is associated with neurite outgrowth. However, neurite outgrowth resistance occurred when PC12 Adh cell was treated with ROCK inhibitors for a longer time. PC12 Adh cells were treated with ROCK inhibitor Y27632 or NGF for different durations. Neurite outgrowth resistance occurred when PC12 Adh cell exposed to Y27632 (33 µM) for 3 or more days, but not happen when exposed to nerve growth factor (NGF, 100 ng/mL). The gene expression in the PC12 Adh cells treated with Y27632 (33 µM) or NGF (100 ng/mL) for 2 or 4 days was assayed by gene microarray, and the reliability of the results were confirmed by real-time RT-PCR. Cluster analysis proved that the gene expression profile of PC12 Adh cell treated with Y27632 for 4 days was different from that treated with Y27632 for 2 days and those treated with NGF for 2 and 4 days, respectively. Pathway analysis hinted that the neurite outgrowth resistance could be associated with up-regulation of inflammatory pathways, especially rno04610 (complement and coagulation cascades), and down-regulation of cell cycle pathways, especially rno04110.
- Subjects :
- Neurite
Pyridines
Cellular differentiation
Neurogenesis
Cell
Drug Resistance
Gene Expression
Biology
PC12 Cells
Gene expression
Nerve Growth Factor
medicine
Neurites
Animals
Rho-associated protein kinase
rho-Associated Kinases
Gene Expression Profiling
Cell Differentiation
Cell Biology
General Medicine
Cell cycle
Microarray Analysis
Molecular biology
Amides
Rats
medicine.anatomical_structure
Nerve growth factor
Rho kinase inhibitor
Signal Transduction
Subjects
Details
- ISSN :
- 10958355
- Volume :
- 39
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Cell biology international
- Accession number :
- edsair.doi.dedup.....2a5a3a0993e3b115d29d4a818679c678