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Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris
- Source :
- Biotechnology Letters. 35:97-105
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- By predicting the potential signal peptides from proteins that are naturally secreted by Pichia pastoris, we identified three possible endogenous signal peptides: Scw, Dse and Exg. We compared their capability to mediate the secretion of enhanced green fluorescent protein (EGFP) and Candida antarctica lipase B (CALB) with that of the Saccharomyces cerevisiae α-factor prepro-signal. EGFP entered the secretory pathway of P. pastoris and was efficiently secreted into the culture medium by all three endogenous peptides. Further, these three putative endogenous signal peptides were also effective in secreting CALB. These endogenous signal peptides thus have the potential to mediate the efficient secretion of heterologous proteins in P. pastoris.
- Subjects :
- Signal peptide
Green Fluorescent Proteins
Molecular Sequence Data
Saccharomyces cerevisiae
Gene Dosage
Bioengineering
Endogeny
Protein Sorting Signals
Applied Microbiology and Biotechnology
Pichia
Green fluorescent protein
Pichia pastoris
Fungal Proteins
Secretion
Amino Acid Sequence
Cloning, Molecular
Peptide sequence
Secretory pathway
biology
Chemistry
Lipase
General Medicine
biology.organism_classification
Molecular biology
Recombinant Proteins
Microscopy, Fluorescence
Biochemistry
Biotechnology
Subjects
Details
- ISSN :
- 15736776 and 01415492
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Biotechnology Letters
- Accession number :
- edsair.doi.dedup.....2a41c2721f10fd4b6287a162dc84e6c1