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Influence of the difference in refractive index on the interface of an object and the surroundings in near-infrared fluorescence tomography

Authors :
Maho Takematsu
Masakazu Umezawa
Toshihiro Sera
Yuichi Kitagawa
Hiroyuki Kurahashi
Shuta Yamada
Kyohei Okubo
Masao Kamimura
Hideo Yokota
Kohei Soga
Source :
Applied Optics. 61(No. 2):638
Publication Year :
2022

Abstract

The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest fluorescent point is away from the correct point on the tomograms owing to the refraction. The speculated position is determined as the exact point if the RI ratio ranges between 0.97 and 1.03 by immersing the body in an RI matching liquid. The results can help in experimental settings of fluorescence CT for acquiring three-dimensional positional information.

Details

Language :
English
ISSN :
1559128X
Volume :
61
Issue :
No. 2
Database :
OpenAIRE
Journal :
Applied Optics
Accession number :
edsair.doi.dedup.....2a11d62d370c0bdcb3f18e972399d0c6