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Defective expansion and function of memory like natural killer cells in HIV+ individuals with latent tuberculosis infection
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 9, p e0257185 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science, 2021.
-
Abstract
- PurposeTuberculosis (TB) is the leading cause of infectious disease related mortality, and only 10% of the infected individuals develop active disease. The likelihood of progression of latent tuberculosis infection (LTBI) to active TB disease is high in HIV infected individuals. Identification of HIV+ individuals at risk would allow treating targeted population, facilitating completion of therapy for LTBI and prevention of TB development. NK cells have an important role in T cell independent immunity against TB, but the exact role of NK cell subsets in LTBI and HIV is not well characterized.MethodsIn this study, we compared the expansion and function of memory like NK cells from HIV-LTBI+ individuals and treatment naïve HIV+LTBI+ patients in response to Mtb antigens ESAT-6 and CFP-10.ResultsIn freshly isolated PBMCs, percentages of CD3-CD56+NK cells were similar in HIV+LTBI+ patients and healthy HIV-LTBI+ individuals. However, percentages of CD3-CD56+CD16+NK cells were higher in healthy HIV-LTBI+ individuals compared to HIV+LTBI+ patients. HIV infection also inhibited the expansion of memory like NK cells, production of IL-32α, IL-15 and IFN-γ in response to Mtb antigens in LTBI+ individuals.ConclusionWe studied phenotypic, functional subsets and activation of memory like-NK cells during HIV infection and LTBI. We observed that HIV+LTBI+ patients demonstrated suboptimal NK cell and monocyte interactions in response to Mtb, leading to reduced IL-15, IFN-γ and granzyme B and increased CCL5 production. Our study highlights the effect of HIV and LTBI on modulation of NK cell activity to understand their role in development of interventions to prevent progression to TB in high risk individuals.
- Subjects :
- Bacterial Diseases
RNA viruses
Physiology
HIV Infections
NK cells
Cell Communication
Pathology and Laboratory Medicine
Monocytes
Granzymes
Spectrum Analysis Techniques
Immunodeficiency Viruses
Immune Physiology
Cellular types
Interleukin-15
education.field_of_study
Innate Immune System
Multidisciplinary
biology
Latent tuberculosis
Immune cells
virus diseases
Flow Cytometry
Actinobacteria
Killer Cells, Natural
medicine.anatomical_structure
Medical Microbiology
Spectrophotometry
Viral Pathogens
Viruses
Medicine
White blood cells
Infectious diseases
Cytokines
Cytophotometry
Pathogens
Chemokines
Research Article
Medical conditions
Adult
Cell biology
Blood cells
Tuberculosis
T cell
Science
Population
Immunology
Viral diseases
Research and Analysis Methods
Microbiology
CCL5
Mycobacterium tuberculosis
Interferon-gamma
Antigen
Latent Tuberculosis
Retroviruses
medicine
Humans
education
Microbial Pathogens
Cell Proliferation
Medicine and health sciences
Biology and life sciences
Bacteria
business.industry
Interleukins
Lentivirus
Organisms
HIV
Molecular Development
biology.organism_classification
medicine.disease
bacterial infections and mycoses
Tropical Diseases
Lymphocyte Subsets
Granzyme B
Animal cells
Immune System
business
Immunologic Memory
Mycobacterium Tuberculosis
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....2a05aae592cda901f78eb23f87cd5f55