Back to Search
Start Over
Plasmalogen degradation by oxidative stress: production and disappearance of specific fatty aldehydes and fatty alpha-hydroxyaldehydes
- Source :
- Free radical biologymedicine. 31(10)
- Publication Year :
- 2001
-
Abstract
- Plasmalogens are often considered as antioxidant molecules that protect cells from oxidative stress. Their vinyl ether bond could indeed be among the first targets for newly formed radicals. However, the long chain aldehydes released from plasmalogens were seldom studied and possible injurious or harmless effects were poorly examined. Thus, the sensitivity of the vinyl ether bond of plasmalogens was investigated in a cerebral cortex homogenate under UV irradiation- or Fe2+/ascorbate-induced peroxidation. Kinetics of aldehyde production was followed by gas chromatography/mass spectrometry. This confirmed that plasmalogens were highly sensitive to oxidative stress (70% cleavage after 90 min UV irradiation and 30% after 30 min of Fe2+/ascorbate). The aldehydes corresponding to sn-1 position 16:0, 18:0, or 18:1 were poorly detected. Conversely, oxidation of plasmalogens yielded preferentially 15:0, 17:0, and 17:1 aldehydes under UV and the alpha-hydroxyaldehydes 16:0-OH and 18:0-OH following a Fe2+/ascorbate oxidation. Kinetics showed that free aldehydes and above all free alpha-hydroxyaldehydes disappeared from the medium as soon as produced. Consequently, the behavior of these released aldehydes in the tissues has to be investigated in order to ascertain the protective effect of plasmalogens against oxidation.
- Subjects :
- Male
Antioxidant
Plasmalogen
Radical
medicine.medical_treatment
Kinetics
Plasmalogens
In Vitro Techniques
medicine.disease_cause
Biochemistry
Aldehyde
Gas Chromatography-Mass Spectrometry
chemistry.chemical_compound
Physiology (medical)
medicine
Animals
Rats, Wistar
chemistry.chemical_classification
Cerebral Cortex
Aldehydes
Singlet Oxygen
Chemistry
Hydroxyl Radical
Fatty Acids
Vinyl ether
Rats
Oxidative Stress
Hydroxyl radical
Oxidation-Reduction
Oxidative stress
medicine.drug
Subjects
Details
- ISSN :
- 08915849
- Volume :
- 31
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Free radical biologymedicine
- Accession number :
- edsair.doi.dedup.....29f35af6bd22e95a2d9d3ad6db185405