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Use of oligonucleotide probes to analyse the homology of the oprF gene among clinical and heterologous immunotype strains of Pseudomonas aeruginosa
- Source :
- Molecular and Cellular Probes. 8:395-400
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- The conservation of opr F gene among 25 clinical Pseudomonas aeruginosa strains and a set of 17 serotype-specific representative strains of the international antigen typing scheme (IATS) was analysed by dot-blotting using five specific oligonucleotide probes. The oligo 1, 2, 3, 4, 5 correspond to five different regions of the opr F gene and hybridized strongly with respectively 88%, 88%, 76%, 94% and 71% of the IATS strains and 88%, 96%, 92%, 88% and 92% of the clinical strains. A parallel study performed with the whole opr F gene showed a lack of specificity of this probe: indeed, the probe hybridized not only with the 42 Pseudomonas aeruginosa strains but also with Escherichia coli and Salmonella minnesota . This study suggests that the gene sequence encoding the protein F is not totally conserved among Pseudomonas aeruginosa strains.
- Subjects :
- Salmonella minnesota
Base Sequence
Pseudomonas aeruginosa
Oligonucleotide
Molecular Sequence Data
Porins
Heterologous
Cell Biology
Biology
medicine.disease_cause
Molecular biology
Homology (biology)
Microbiology
Species Specificity
Genes, Bacterial
Sequence Homology, Nucleic Acid
Antigen typing
medicine
Serotyping
Oligonucleotide Probes
Molecular Biology
Escherichia coli
Gene
Conserved Sequence
Subjects
Details
- ISSN :
- 08908508
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Probes
- Accession number :
- edsair.doi.dedup.....29c8c8967bc2c21e00f2b49f3ce8fc94