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Grim-19 expressed by recombinant adenovirus for esophageal neoplasmtarget therapy
- Source :
- Molecular Medicine Reports.
- Publication Year :
- 2018
- Publisher :
- Spandidos Publications, 2018.
-
Abstract
- Esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EA) are the two most common types of esophageal cancer, which is the sixth highest cause of cancer‑associated mortality and the eighth most common cancer worldwide. Gene associated with retinoid‑interferon (IFN)‑induced mortality‑19 (Grim‑19) is reported to be a cell death activator that may be used to define mechanisms involved in IFN‑β‑ and retinoic acid‑induced cell death and apoptosis in a number of tumor cell lines. The present study constructed a recombinant adenovirus expressing Grim‑19 (rAd‑Grim‑19) and investigated its therapeutic outcomes in ESCC cells and tumor‑bearing mice. Grim‑19 expression was detected in EC‑109 (ESCC) cells by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Tumor cell death and apoptosis induced by rAd‑Grim‑19 in EC‑109 cells were analyzed by flow cytometry. The inhibitory effects of rAd‑Grim‑19 on EC‑109 growth were determined by MTT assays. Furthermore, the therapeutic effects of rAd‑Grim‑19 were investigated in EC‑109‑bearing mice. The results demonstrated that Grim‑19 mRNA and protein expression was downregulated in EC‑109 esophageal carcinoma cells compared with Het‑1A normal esophageal epithelial cells. In addition, EC‑109 cells exhibited a significant reduction in tumor cell growth in the rAd‑Grim‑19 group compared with the control groups. Furthermore, rAd‑Grim‑19 increased EC‑109 cell apoptosis compared with the control group. These results indicated that rAd-Grim-19 may regulate tumor cell growth and apoptosis. Additionally, the results demonstrated that rAd‑Grim‑19 led to beneficial outcomes and prolonged the survival of esophageal tumor‑bearing mice. In conclusion, the present study demonstrated that rAd‑Grim‑19 may have potential as an antitumor agent for esophageal neoplasms and may therefore be beneficial for patients with esophageal neoplasms.
- Subjects :
- Male
0301 basic medicine
Cancer Research
Esophageal Neoplasm
Programmed cell death
animal structures
Esophageal Neoplasms
Cell
Mice, Nude
Apoptosis
Biochemistry
Adenoviridae
Mice
03 medical and health sciences
Transduction, Genetic
Cell Line, Tumor
Genetics
Carcinoma
Animals
Humans
Medicine
NADH, NADPH Oxidoreductases
Molecular Biology
Mice, Inbred BALB C
Oncogene
business.industry
fungi
Middle Aged
Esophageal cancer
Cell cycle
medicine.disease
Xenograft Model Antitumor Assays
body regions
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
Oncology
Cancer research
Molecular Medicine
Female
Apoptosis Regulatory Proteins
business
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....29c837b9b9290c70c483205d615173fd