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Imaging the beating heart in the mouse using intravital microscopy techniques
- Source :
- Nature Protocols. 10:1802-1819
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Real-time microscopic imaging of moving organs at single-cell resolution represents a major challenge in studying complex biology in living systems. Motion of the tissue from the cardiac and respiratory cycles severely limits intravital microscopy by compromising ultimate spatial and temporal imaging resolution. However, significant recent advances have enabled single-cell resolution imaging to be achieved in vivo. In this protocol, we describe experimental procedures for intravital microscopy based on a combination of thoracic surgery, tissue stabilizers and acquisition gating methods, which enable imaging at the single-cell level in the beating heart in the mouse. Setup of the model is typically completed in 1 h, which allows 2 h or more of continuous cardiac imaging. This protocol can be readily adapted for the imaging of other moving organs, and it will therefore broadly facilitate in vivo high-resolution microscopy studies.
- Subjects :
- Beating heart
Intravital Microscopy
business.industry
Myocardium
Optical Imaging
Resolution (electron density)
Heart
Image processing
Anatomy
Article
General Biochemistry, Genetics and Molecular Biology
Mice
Computer Systems
In vivo
Microscopy
Microscopic imaging
Animals
Medicine
business
Intravital microscopy
Cardiac imaging
Biomedical engineering
Subjects
Details
- ISSN :
- 17502799 and 17542189
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Nature Protocols
- Accession number :
- edsair.doi.dedup.....29c112e544456ca96b5a7344822b1f2e