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Cloning and characterization of nanos gene in silkworm Bombyx mori
- Source :
- Journal of genetics and genomics = Yi chuan xue bao. 35(2)
- Publication Year :
- 2007
-
Abstract
- Gene nanos is a maternal posterior group gene required for normal development of abdominal segments and the germ line in Drosophila. Expression of nanos-related genes is associated with the germ line in a broad variety of other taxa. In this study, the 5'-RACE method and the in silico cloning method are used to isolate the new nanos-like gene of Bombyx mori and the gene obtained is analyzed with bioinformatics tools. The putative protein is expressed in Escherichia coli and the antiserum has been produced in New Zealand white rabbits. The result shows that the nanos cDNA is 1,913 bp in full length and contains a 954 bp open reading frame. The deduced protein has 317 amino acid residues, with a predicted molecular weight of 35 kDa, isoelectric point of 5. 38, and contains a conserved nanos RNA binding domain. The conserved region of the deduced protein shares 73% homology with the nanos protein conserved region of Honeybee (Apis mellifera). This gene has been registered in the GenBank under the accession number EF647589. One encoding sequence of the nanos fragment has been successfully expressed in E. coli. Western blotting analysis indicates that homemade antiserum can specifically detect nanos protein expressed in prokaryotic cells.
- Subjects :
- DNA, Complementary
Molecular Sequence Data
Sequence alignment
Biology
Homology (biology)
Conserved sequence
Complementary DNA
Genetics
Escherichia coli
Animals
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Gene
Peptide sequence
Conserved Sequence
Phylogeny
Expressed Sequence Tags
Expressed sequence tag
Base Sequence
Immune Sera
technology, industry, and agriculture
Computational Biology
Bombyx
Molecular biology
Protein Structure, Tertiary
Open reading frame
Insect Proteins
Sequence Alignment
Transcription Factors
Subjects
Details
- ISSN :
- 16738527
- Volume :
- 35
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of genetics and genomics = Yi chuan xue bao
- Accession number :
- edsair.doi.dedup.....29b0d58491dd3ef354f936815f1f88e1