Back to Search
Start Over
Epstein-Barr virus DNA is not increased in tonsillar carcinoma
- Source :
- The Laryngoscope. 111(5)
- Publication Year :
- 2001
-
Abstract
- Epstein-Barr virus (EBV) is a known oncogenic virus associated with a wide variety of cancers, including nasopharyngeal carcinoma. Waldeyer's ring, a collection of lymphoid tissues, includes the nasopharynx, pharyngeal, and lingual tonsils. To determine if EBV plays a causative role in carcinomas arising from other tissues in Waldeyer's ring, we examined pharyngeal tonsillar carcinomas for evidence of EBV infection. As previously reported, DNA was extracted from 53 consecutive tonsil cancers, as well as from age- and gender-matched non-cancerous tonsillectomy specimens. Three different sets of primers for discrete exons of EBV were then used to determine if active or latent EBV infection was expressed in the extracted DNA using the polymerase chain reaction (PCR). All positive bands were then sequenced to confirm the presence of amplified EBV fragments. None of the samples showed evidence for active EBV infection. In primers demonstrating latent infection, 1 of 53 (1.9%) of tumors were positive, versus 6 of 53 (11.3%) of the controls. These results indicate that EBV expression is not increased in DNA from tonsil cancers and that EBV infection does not have a causal relationship with tonsil cancer.
- Subjects :
- Male
Tonsillar Carcinoma
Epstein-Barr Virus Infections
Herpesvirus 4, Human
Tonsillar Neoplasms
medicine.disease_cause
Polymerase Chain Reaction
Herpesviridae
law.invention
stomatognathic system
law
hemic and lymphatic diseases
medicine
Gammaherpesvirinae
Humans
Polymerase chain reaction
biology
Middle Aged
biology.organism_classification
medicine.disease
Epstein–Barr virus
Virology
medicine.anatomical_structure
Otorhinolaryngology
Nasopharyngeal carcinoma
Tonsil
DNA, Viral
Carcinoma, Squamous Cell
Female
Oncovirus
Subjects
Details
- ISSN :
- 0023852X
- Volume :
- 111
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Laryngoscope
- Accession number :
- edsair.doi.dedup.....29ad0e4140b3e2335c692524d129a633