Chalcones and their B-aryl analogues as myeloperoxidase inhibitors: In silico, in vitro and ex vivo investigations

Made available in DSpace on 2021-06-25T10:55:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) In the present study, a series of chalcones and their B-aryl analogues were prepared and evaluate as inhibitors of myeloperoxidase (MPO) chlorinating activity, using in vitro and ex vivo assays. Among these, B-thiophenyl chalcone (analogue 9) demonstrated inhibition of in vitro and ex vivo MPO chlorinating activity, exhibiting IC50 value of 0.53 and 19.2 µM, respectively. Potent ex vivo MPO inhibitors 5, 8 and 9 were not toxic to human neutrophils at 50 µM, as well as displayed weak 2,2-diphenyl-1-pycrylhydrazyl radical (DPPH•) and hypochlorous acid (HOCl) scavenger abilities. Docking simulations indicated binding mode of MPO inhibitors, evidencing hydrogen bonds between the amino group at 4′position (ring A) of chalcones with Gln91, Asp94, and Hys95 MPO residues. In this regard, the efficacy and low toxicity promoted aminochalcones and arylic analogues to the rank of hit compounds in the search for new non-steroidal anti-inflammatory compounds. Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP) Department of Chemistry Faculty of Sciences São Paulo State University (UNESP) Physics Institute of São Carlos University of São Paulo Department of Organic Chemistry Institute of Chemistry São Paulo State University Department of Chemistry State University of Londrina (UEL) Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP) Department of Chemistry Faculty of Sciences São Paulo State University (UNESP) Department of Organic Chemistry Institute of Chemistry São Paulo State University