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Nerve growth factor is expressed and stored in central neurons of adult zebrafish
- Source :
- J Anat, Journal of Anatomy, Journal of Anatomy, Wiley, 2019, 325 (1), pp.167-179. ⟨10.1111/joa.12986⟩, Journal of Anatomy, 2019, 325 (1), pp.167-179. ⟨10.1111/joa.12986⟩
- Publication Year :
- 2019
- Publisher :
- John Wiley and Sons Inc., 2019.
-
Abstract
- International audience; Nerve growth factor (NGF), a member of the neurotrophin family, was initially described as neuronal survival and growth factor, but successively has emerged as an active mediator in many essential functions in the central nervous system of mammals. NGF is synthesized as a precursor pro-NGF and is cleaved intracellularly into mature NGF. However, recent evidence demonstrates that pro-NGF is not a simple inactive precursor, but is also secreted outside the cells and can exert multiple roles. Despite the vast literature present in mammals, studies devoted to NGF in the brain of other vertebrate models are scarce. Zebrafish is a teleost fish widely known for developmental genetic studies and is well established as model for translational neuroscience research. Genomic organization of zebrafish and mouse NGF is highly similar, and zebrafish NGF protein has been reported in mature and two-precursors forms. To add further knowledge on neurotrophic factors in vertebrate brain models, we decided to determine the NGF mRNA and protein distribution in the adult zebrafish brain and to characterize the phenotype of NGF-positive cells. NGF mRNA was visualized by in situ hybridization on whole-mount brains. NGF protein distribution was assessed on microtomic sections by using an antiserum against NGF, able to recognize pro-NGF in adult zebrafish brain as demonstrated also in previous studies. To characterize NGF-positive cells, anti-NGF was employed on microtomic slides of aromatase B transgenic zebrafish (where radial glial cells appeared fluorescent) and by means of double-immunolabeling against NGF/proliferative cell nuclear antigen (PCNA; proliferation marker) and NGF/microtube-associated protein2 (MAP2; neuronal marker). NGF mRNA and protein were widely distributed in the brain of adult zebrafish, and their pattern of distribution of positive perikaryal was overlapping, both in males and females, with few slight differences. Specifically, the immunoreactivity to the protein was observed in fibers over the entire encephalon. MAP2 immunoreactivity was present in the majority of NGF-positive cells, throughout the zebrafish brain. PCNA and aromatase B cells were not positive to NGF, but they were closely intermingled with NGF cells. In conclusion, our study demonstrated that mature neurons in the zebrafish brain express NGF mRNA and store pro-NGF.
- Subjects :
- 0301 basic medicine
Central Nervous System
Male
Histology
medicine.medical_treatment
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Central nervous system
neurotrophins
03 medical and health sciences
0302 clinical medicine
Neurotrophic factors
Nerve Growth Factor
medicine
Animals
Proliferation Marker
encephalon
Nerve Growth Factors
RNA, Messenger
Aromatase
Molecular Biology
Zebrafish
Ecology, Evolution, Behavior and Systematics
In Situ Hybridization
fish
Neurons
biology
Growth factor
neurotrophin
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Brain
Cell Biology
Original Articles
biology.organism_classification
Cell biology
030104 developmental biology
Nerve growth factor
medicine.anatomical_structure
nervous system
biology.protein
Female
Anatomy
030217 neurology & neurosurgery
Developmental Biology
Neurotrophin
zebrafish neurons
Subjects
Details
- Language :
- English
- ISSN :
- 00218782 and 14697580
- Database :
- OpenAIRE
- Journal :
- J Anat, Journal of Anatomy, Journal of Anatomy, Wiley, 2019, 325 (1), pp.167-179. ⟨10.1111/joa.12986⟩, Journal of Anatomy, 2019, 325 (1), pp.167-179. ⟨10.1111/joa.12986⟩
- Accession number :
- edsair.doi.dedup.....2993df6a3802f611c58c89a9e5af3b19