Back to Search
Start Over
The Ferret as a Surgical Model for Vocal Fold Scar Creation and Treatment
- Source :
- Annals of Otology, Rhinology & Laryngology. 127:146-154
- Publication Year :
- 2018
- Publisher :
- SAGE Publications, 2018.
-
Abstract
- Objectives: To develop a vocal fold (VF) scarring procedure in the ferret, characterize the scars histologically, and test the injectability of the lamina propria (LP). Secondarily, to compare laryngeal anatomy of the ferret with rat and rabbit. Materials and Methods: The larynges of 18 male ferrets were prepared by unilateral scarring, and normal larynges from 6 female Wistar rats and 5 male albino rabbits were used for comparative purposes. For scarring, the right VF were electrocauterized, ablating the entire LP. Prior to harvesting the larynges at 4 and 16 weeks, each ferret was re-anesthetized, and in 3 animals, India ink was injected into the LPs of both normal and scarred VFs. Results: Laryngoscopic methods and instrumentation for precise visualization, scarring, and injection were developed. The scarred VFs had reduced hyaluronic acid and increased collagen type I, III, and fibronectin compared with normal VFs. The 2 timepoints (4 and 16 weeks) differed significantly only in collagen type III level (levels were higher at 4 weeks). Injected ink migrated from scarred LP to muscle layer just beneath the scarred tissue 3 hours after injection. Conclusion: The ferret is a promising species for creation and experimental treatment of vocal fold scar.
- Subjects :
- Models, Anatomic
Pathology
medicine.medical_specialty
Scars
Vocal Cords
Collagen Type I
Cicatrix
03 medical and health sciences
Collagen Type III
chemistry.chemical_compound
0302 clinical medicine
Animal model
Hyaluronic acid
Electrocoagulation
Animals
Medicine
Laryngeal anatomy
030223 otorhinolaryngology
Collagen type
Lamina propria
Mucous Membrane
Laryngoscopy
business.industry
Ferrets
General Medicine
India ink
Fibronectins
Rats
medicine.anatomical_structure
Otorhinolaryngology
chemistry
030220 oncology & carcinogenesis
Female
Rabbits
medicine.symptom
business
Subjects
Details
- ISSN :
- 1943572X and 00034894
- Volume :
- 127
- Database :
- OpenAIRE
- Journal :
- Annals of Otology, Rhinology & Laryngology
- Accession number :
- edsair.doi.dedup.....297762e325728c120be7e06b04a4bfe0