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Mobilization of retrotransposons in synthetic allotetraploid tobacco
- Source :
- New Phytologist, New Phytologist, Wiley, 2010, 186 (1), pp.135-147. ⟨10.1111/j.1469-8137.2009.03140.x⟩, New Phytologist 1 (186), 135-147. (2010)
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- Allopolyploidy is a major driving force in plant evolution and can induce rapid structural changes in the hybrid genome. As major components of plant genomes, transposable elements are involved in these changes. In a previous work, we observed turnover of retrotransposon insertions in natural allotretraploid tobacco (Nicotiana tabacum). Here, we studied the early stages of allopolyploid formation by monitoring changes at retrotransposon insertion sites in the Th37 synthetic tobacco. • We used sequence-specific amplification polymorphism (SSAP) to study insertion patterns of two populations of the Tnt1 retrotransposon in Th37 S4 generation plants, and characterized the nature of polymorphic insertion sites. • We observed significant amplification of young Tnt1 populations. Newly transposed copies were amplified from maternal elements and were highly similar to Tnt1A tobacco copies amplified in response to microbial factors. A high proportion of paternal SSAP bands were not transmitted to the hybrid, corresponding to various rearrangements at paternal insertion sites, including indels or the complete loss of the Tnt1/flanking junction. • These data indicate that major changes, such as retrotransposon amplification and molecular restructuring in or around insertion sites, occur rapidly in response to allopolyploidy.
- Subjects :
- 0106 biological sciences
Physiology
Nicotiana tabacum
tnt1
Retrotransposon
Plant Science
sequence-specific
01 natural sciences
Genome
allopolyploidy
génétique
stress
Chromosome Segregation
chromosome
évolution
Phylogeny
2. Zero hunger
Plant evolution
Genetics
0303 health sciences
biology
food and beverages
expression des gènes
Transposable element
Retroelements
Phytopathology and phytopharmacy
Molecular Sequence Data
amplification polymorphism (SSAP)
Polyploidy
03 medical and health sciences
Tobacco
evolution
Indel
Gene
Crosses, Genetic
retrotransposon
nicotiana
030304 developmental biology
Nicotiana
Polymorphism, Genetic
Base Sequence
génome
fungi
biology.organism_classification
Phytopathologie et phytopharmacie
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
tabac
Mutagenesis, Insertional
Sequence Alignment
nicotine
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 0028646X and 14698137
- Database :
- OpenAIRE
- Journal :
- New Phytologist, New Phytologist, Wiley, 2010, 186 (1), pp.135-147. ⟨10.1111/j.1469-8137.2009.03140.x⟩, New Phytologist 1 (186), 135-147. (2010)
- Accession number :
- edsair.doi.dedup.....297535674bf5a2adfc473216f54e360d