Back to Search
Start Over
Simultaneous Structural Identification of Natural Products in Fractions of Crude Extract of the Rare Endangered Plant Anoectochilus roxburghii Using 1H NMR/RRLC-MS Parallel Dynamic Spectroscopy
- Source :
- International Journal of Molecular Sciences; Volume 12; Issue 4; Pages: 2556-2571, International Journal of Molecular Sciences, Vol 12, Iss 4, Pp 2556-2571 (2011)
- Publication Year :
- 2011
- Publisher :
- MDPI AG, 2011.
-
Abstract
- Nuclear magnetic resonance/liquid chromatography-mass spectroscopy parallel dynamic spectroscopy (NMR/LC-MS PDS) is a method aimed at the simultaneous structural identification of natural products in complex mixtures. In this study, the method is illustrated with respect to 1H NMR and rapid resolution liquid chromatography-mass spectroscopy (RRLC-MS) data, acquired from the crude extract of Anoectochilus roxburghii, which was separated into a series of fractions with the concentration of constituent dynamic variation using reversed-phase preparative chromatography. Through fraction ranges and intensity changing profiles in 1H NMR/RRLC–MS PDS spectrum, 1H NMR and the extracted ion chromatogram (XIC) signals deriving from the same individual constituent, were correlated due to the signal amplitude co-variation resulting from the concentration variation of constituents in a series of incompletely separated fractions. 1H NMR/RRLC-MS PDS was then successfully used to identify three types of natural products, including eight flavonoids, four organic acids and p-hydroxybenzaldehyde, five of which have not previously been reported in Anoectochilus roxburghii. In addition, two groups of co-eluted compounds were successfully identified. The results prove that this approach should be of benefit in the unequivocal structural determination of a variety of classes of compounds from extremely complex mixtures, such as herbs and biological samples, which will lead to improved efficiency in the identification of new potential lead compounds.
- Subjects :
- Dynamic spectroscopy
Resolution (mass spectrometry)
Analytical chemistry
Fraction (chemistry)
crude extract
Mass spectrometry
Catalysis
lcsh:Chemistry
Inorganic Chemistry
1H NMR/RRLC-MS parallel dynamic spectroscopy
organic acids
Physical and Theoretical Chemistry
Spectroscopy
Anoectochilus roburghii
lcsh:QH301-705.5
Molecular Biology
Chromatography
Chemistry
Organic Chemistry
flavonoids
General Medicine
Nuclear magnetic resonance spectroscopy
Computer Science Applications
lcsh:Biology (General)
lcsh:QD1-999
Anoectochilus roxburghii
Proton NMR
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....29714ed8b47a1632d0763be6c673a8be