TNFA and IL10 Gene Polymorphisms are not Associated with Periodontitis in Brazilians

IL-10 and TNF-α are cytokines that have complex and opposing roles in the inflammatory responses. G/A polymorphisms at position –1082 ofIL10and –308 ofTNFAgenes have been reported to influence the expression of IL-10 and TNF-α, respectively. The aim of this study was to investigate the association between theIL10(-1082) andTNFA(- 308) gene polymorphisms with different clinical forms or severity of periodontitis in a sample of Brazilian individuals. DNA was obtained from oral swabs of 165 Brazilian individuals, which were divided into three groups: individuals with chronic periodontitis, aggressive periodontitis and individuals without clinical evidence of periodontitis. Evaluation ofIL10andTNFApolymorphisms was performed by RFLP analysis. Statistical analysis of data was performed using the χ2likelihood ratio and Fisher`s exact test. No significant differences in the genotype and allele distribution of eitherIL10orTNFAwere observed among individuals with different clinical forms or with different degrees of severity of periodontitis. Moreover, combined analysis ofIL10andTNFApolymorphisms did not show any association with periodontal status. As conclusion, theIL10andTNFAgene promoter polymorphisms investigated are not associated with different clinical forms of periodontitis or with severity of the disease in the Brazilian population polymorphisms.