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Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry
- Source :
- Annals of the New York Academy of Sciences
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- Newly emergent infectious diseases are a global public health problem. The population dense regions of Southeast Asia are the epicenter of many emerging diseases, as evidenced by the outbreak of Nipah, SARS, avian influenza (H5N1), Dengue, and enterovirus 71 in this region in the past decade. Rapid identification, epidemiologic surveillance, and mitigation of transmission are major challenges in ensuring public health safety. Here we describe a powerful new approach for infectious disease surveillance that is based on polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry (ESI‐MS) for accurate mass measurements of the PCR products, and base composition signature analysis to identify organisms in a sample. This approach is capable of automated analysis of more than 1,500 PCR reactions a day. It is applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens and will facilitate rapid characterization of known and emerging pathogens.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
RT‐PCR
broad detection
ESI‐MS
Electrospray ionization
Population
Biology
infectious disease surveillance
medicine.disease_cause
Communicable Diseases, Emerging
Polymerase Chain Reaction
General Biochemistry, Genetics and Molecular Biology
law.invention
History and Philosophy of Science
law
medicine
Enterovirus 71
Humans
education
Polymerase chain reaction
mass spectrometry
education.field_of_study
viral diseases
emerging infections
General Neuroscience
Outbreak
Original Articles
Epidemiologic Surveillance
biology.organism_classification
Virology
Influenza A virus subtype H5N1
PCR
Virus Diseases
Infectious disease (medical specialty)
Communicable Disease Control
Viruses
Subjects
Details
- ISSN :
- 17496632 and 00778923
- Volume :
- 1102
- Database :
- OpenAIRE
- Journal :
- Annals of the New York Academy of Sciences
- Accession number :
- edsair.doi.dedup.....294d5c9cdc74c5c753e7cb3b9bf119d7