Back to Search
Start Over
Phosphorylated Alpha-Synuclein Within Cutaneous Autonomic Nerves of Patients With Parkinson’s Disease: The Implications of Sample Thickness on Results
- Source :
- J Histochem Cytochem
- Publication Year :
- 2020
- Publisher :
- SAGE Publications, 2020.
-
Abstract
- The detection of cutaneous phosphorylated alpha-synuclein (P-syn) in patients with Parkinson’s disease (PD) has ranged from 30% to 100% across different studies. We hypothesize that part of the variability in P-syn detection is due to methodological differences using sections of different tissue thickness. Three skin biopsies were obtained from 29 individuals with PD and 21 controls. Tissues were cut into 10-, 20-, and 50-µm-thick sections and double-stained with protein gene product (PGP) 9.5 and P-syn. We quantified the deposition of P-syn with and without PGP 9.5 in sweat glands, pilomotor muscle, and blood vessels using confocal digital images of autonomic structures. Overall, the P-syn-positive rates with PGP 9.5 colocalization in subjects with PD were 100% using 50 µm sections, 90% using 20 µm sections, and 73% using 10 µm sections with 100% specificity. (No P-syn was detected within control subjects.) Without PGP 9.5, colocalization of the P-syn-positive rates was 100% for all samples, but specificity dropped below 70%. In this study, double-immunostained 50 µm skin biopsy tissue sections are superior to 20 and 10 µm tissue sections at detecting P-syn in subjects with PD. The increased sensitivity is likely secondary to a combination of greater volume of tissue analyzed and improved visualization of nerve fiber architecture.
- Subjects :
- Male
0301 basic medicine
Pathology
medicine.medical_specialty
Histology
Parkinson's disease
Confocal
Nerve fiber
SWEAT
Gene product
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Humans
Autonomic Pathways
Phosphorylation
Aged
Alpha-synuclein
medicine.diagnostic_test
business.industry
Colocalization
Parkinson Disease
Articles
Middle Aged
medicine.disease
030104 developmental biology
medicine.anatomical_structure
chemistry
Skin biopsy
alpha-Synuclein
Female
Anatomy
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15515044 and 00221554
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Journal of Histochemistry & Cytochemistry
- Accession number :
- edsair.doi.dedup.....292d67598e82e4a24f796343e0d122d1