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MicroRNA hsa-miR-134 is a circulating biomarker for mesial temporal lobe epilepsy
- Source :
- PLoS ONE, Vol 12, Iss 4, p e0173060 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Epilepsy is misdiagnosed in up to 25% of patients, leading to serious and long-lasting consequences. Recently, circulating microRNAs have emerged as potential biomarkers in a number of clinical scenarios. The purpose of this study was to identify and to validate circulating microRNAs that could be used as biomarkers in the diagnosis of epilepsy. Quantitative real-time PCR was used to measure plasma levels of three candidate microRNAs in two phases of study: an initial discovery phase with 14 patients with mesial temporal lobe epilepsy (MTLE), 13 with focal cortical dysplasia (FCD) and 16 controls; and a validation cohort constituted of an independent cohort of 65 patients with MTLE and 83 controls. We found hsa-miR-134 downregulated in patients with MTLE (p = 0.018) but not in patients with FCD, when compared to controls. Furthermore, hsa-miR-134 expression could be used to discriminate MTLE patients with an area under the curve (AUC) of 0.75. To further assess the robustness of hsa-miR-134 as a biomarker for MTLE, we studied an independent cohort of 65 patients with MTLE, 27 of whom MTLE patients were responsive to pharmacotherapy, and 38 patients were pharmacoresistant and 83 controls. We confirmed that hsa-miR-134 was significantly downregulated in the plasma of patients with MTLE when compared with controls (p < 0.001). In addition, hsa-miR-134 identified patients with MTLE regardless of their response to pharmacotherapy or the presence of MRI signs of hippocampal sclerosis. We revealed that decreased expression of hsa-miR-134 could be a potential non-invasive biomarker to support the diagnosis of patients with MTLE.
- Subjects :
- Male
0301 basic medicine
Oncology
Physiology
lcsh:Medicine
Biochemistry
Diagnostic Radiology
Cohort Studies
Epilepsy
0302 clinical medicine
Temporal Lobe Epilepsy
Medicine and Health Sciences
lcsh:Science
Multidisciplinary
medicine.diagnostic_test
Pharmaceutics
Radiology and Imaging
Area under the curve
Magnetic Resonance Imaging
Body Fluids
Nucleic acids
Blood
Neurology
Cohort
Biomarker (medicine)
Female
Anatomy
Research Article
Cohort study
medicine.medical_specialty
Imaging Techniques
Research and Analysis Methods
Blood Plasma
03 medical and health sciences
Drug Therapy
Diagnostic Medicine
Internal medicine
Genetics
medicine
Humans
Tonic-Clonic Seizures
Non-coding RNA
Hippocampal sclerosis
Biology and life sciences
business.industry
lcsh:R
Magnetic resonance imaging
Epileptic Seizures
Reverse Transcription
Cortical dysplasia
medicine.disease
Gene regulation
nervous system diseases
MicroRNAs
030104 developmental biology
Epilepsy, Temporal Lobe
RNA
lcsh:Q
Gene expression
business
Biomarkers
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....291b108c1ac9350f10aba30809c8ab7a